NucView® 488 Caspase-3 Enzyme Substrate, 1 mM in DMSO

Biotium products are distributed only in Singapore and Thailand.

Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.

Product Description

NucView® 488 Caspase-3 Substrate detects apoptosis in intact cells based on caspase-3/7 activity using confocal microscopy, flow cytometry, or live cell imaging.

NucView® 488 dye has excitation/emission maxima of 500/530 nm (with DNA)
Rapid, no-wash, endpoint or real-time assays
Non-toxic, allowing multi-day experiments to be performed
For flow cytometry, microscopy or live cell imaging systems
Dual detection of caspase activity and nuclear morphology
Formaldehyde fixable

NucView® substrates consist of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with fluorescence. Thus, NucView caspase-3 substrates are bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView® caspase-3 substrates can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. Staining is compatible with subsequent fixation and permeabilization for immunostaining.

NucView® 488 Caspase-3 Substrate, is also offered as a 1 mM solution in PBS (phosphate-buffered saline) (catalog no. 10403) for use in cells that are sensitive to DMSO toxicity. In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView® 488 staining.

We also offer blue fluorescent NucView® 405 Caspase-3 Substrate for use with the 405 nm laser line, and orange fluorescent NucView® 530 for imaging in the Cy®3 channel.

NucView® enzyme substrate technology is covered by U.S. patents.

Product Attributes

Apoptosis/viability marker	Caspase
For live or fixed cells	For live/intact cells
Assay type/options	Long term staining (24-72h), Endpoint assay, No-wash staining, Real-time imaging, Homogeneous assay
Detection method/readout	Fluorescence microscopy, Live cell imaging, Flow cytometry
Substrate specificity	Caspases
Colors	Green
Fixation options	Fix after staining (formaldehyde), Permeabilize after staining
Excitation/Emission	500/530 nm (with DNA)
Storage Conditions	Store at 2 to 8 °C

Reference Publications

Amit J. Sabnis; Steven Chen; Carolina Morales; Benjamin S. Braun

Abstract A27: An apoptosis-based screen for targeted agents in rhabdomyosarcoma reveals potential combination therapies

Cancer Res 15 July 2020; 80 (14_Supplement): A27.

DOI: https://doi.org/10.1158/1538-7445.PEDCA19-A27

Article Snippet: “We built a screening assay using high-throughput microscopy to quantify both cell number (Hoechst nuclear staining) and apoptosis (NucView 488 Caspase-3 Enzyme Substrate [Biotium]). Technical controls included DMSO and staurosporine.”

Jordan F Hastings, Alvaro Gonzalez Rajal, et al.

Analysis of pulsed cisplatin signalling dynamics identifies effectors of resistance in lung adenocarcinoma

eLife 9:e53367.

DOI: https://doi.org/10.7554/eLife.53367

Article Snippet: Highlight from the figures and data table “Commercial assay or kit NucView 488 Caspase-3 Enzyme substrate Biotium #10402″

Florian Guisier, Pierre Bohn, et al.

In- and ex-vivo molecular imaging of apoptosis to assess sensitivity of non-small cell lung cancer to EGFR inhibitors using probe-based confocal laser endomicroscopy

PLoS ONE 12(7): e0180576.

DOI: https://doi.org/10.1371/journal.pone.0180576

Article Snippet: “The NucView 488 Caspase-3 substrate (Biotium, San Francisco, California, USA), hereafter referred to as C3-NucView, is a fluorogenic dye that detects caspase 3 activity within intact cells, without interfering with the caspase 3 activity.“

Masahiro YAMAMURA, Akira YAMAUCHI, Shigenobu TONE, Yoshiyuki YAMAGUCHI

Sorafenib-induced apoptosis in colonic neuroendocrine carcinoma cells

Kawasaki Medical Journal 48：175－182，

DOI: 10.11482/KMJ-E202248175

Article Snippet: “Caspase-3 activation was analysed using the NucView 488 Caspase-3 Assay Kit for live cells (Biotium, San Francisco, CA, USA), a caspase-3 substrate that fluoresces when cleaved.”

Vasconcelos et al.

Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing

Cell Reports 11, 1474–1485

DOI: http://dx.doi.org/10.1016/j.celrep.2015.05.002

Article Snippet: “For live cell imaging, 1 3 105 pulsed target cells were seeded into microchambers (Lab-Tek Chambered coverglass; Nalgen Nunc) previously coated with poly-D lysine, followed by addition of 2 mM NucView 488 caspase-3 substrate (Biotium)”

Maurice Bosch and Vernonica E. Franklin-Tong

Temporal and spatial activation of caspase-like enzymes induced by self-incompatibility in Papaver pollen

PNAS 104 (46) 18327-18332

DOI: https://doi.org/10.1073/pnas.0705826104

Article Snippet: “The NucView 488 caspase-3 assay Kit (Biotium, Inc., Hayward, CA) was used to detect DEVDase activity in live pollen tubes. The specificity of the DEVDase activity was checked by pretreating hydrated pollen for 1 h with 100 μM Ac-DEVD-CHO or Ac-YVAD-CHO before SI induction.”