Annexin V Conjugates

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Description

Product Description

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

Detect apoptotic cells with fast, reliable detection of phosphatidylserine externalization
Conjugates with wide choice of CF® dye colors, plus biotin, R-PE, APC, and other fluorescent dyes
Biotium’s CF® dyes have superior brightness, photostability, and are water-soluble
Can be used for fluorescence microscopy, live cell imaging, and flow cytometry

CF® Dye, biotin, and FITC conjugates of Annexin V are provided as 50 ug/mL solutions in 10 mM Tris, 1 mM EDTA, 30 mM NaCl, 1 mg/mL bovine serum albumin, 0.1% sodium azide, pH 7.5. R-PE and APC Annexin V conjugates are supplied in PBS, 5 mg/mL BSA, 0.05% sodium azide at 5 uL per test.

Detect Apoptosis

We also offer Annexin V Conjugates, Azide-Free and Lyophilized in colors ranging from UV to far-red for live cell staining, and Near-IR CF® Dye Annexin V (also lyophilized and preservative-free), compatible with small animal NIR in vivo imaging. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Annexin V Conjugates

Product	Ex/Em	Concentration	Size	Catalog No.
Biotin Annexin V	N/A	50 ug/mL	0.5 mL	Purchase 29013
CF®350 Annexin V	347/448 nm	50 ug/mL	0.5 mL	Purchase 29012
CF®405M Annexin V	408/452 nm	50 ug/mL	0.5 mL	Purchase 29009
CF®450 Annexin V	450/538 nm	50 ug/mL	0.5 mL	Purchase 29083
CF®488A Annexin V	490/515 nm	50 ug/mL	0.5 mL	Purchase 29005
CF®555 Annexin V	555/565 nm	50 ug/mL	0.5 mL	Purchase 29004
CF®568 Annexin V	562/583 nm	50 ug/mL	0.5 mL	Purchase 29010
CF®583R Annexin V	586/609 nm	50 ug/mL	0.5 mL	Purchase 29085
CF®594 Annexin V	593/614 nm	50 ug/mL	0.5 mL	Purchase 29011
CF®633 Annexin V	630/650 nm	50 ug/mL	0.5 mL	Purchase 29008
CF®640R Annexin V	642/662 nm	50 ug/mL	0.5 mL	Purchase 29014
CF®647 Annexin V	650/665 nm	50 ug/mL	0.5 mL	Purchase 29003
CF®660R Annexin V	663/682 nm	50 ug/mL	0.5 mL	Purchase 29069
R-PE Annexin V	496, 546, 565/578 nm	5 uL/test	100 uL	Purchase 29045-100uL
R-PE Annexin V	496, 546, 565/578 nm	5 uL/test	500 uL	Purchase 29045-500uL
APC Annexin V	650/660 nm	5 uL/test	100 uL	Purchase 29057-100uL
APC Annexin V	650/660 nm	5 uL/test	500 uL	Purchase 29057-500uL
FITC Annexin V	496/516 nm	50 ug/mL	0.5 mL	Purchase 29001
5X Annexin V Binding Buffer	N/A	5X	15 mL	Purchase 99902

CF is a registered trademark of Biotium, Inc. Alexa Fluor, and DyLight are registered trademarks of Thermo Fisher Scientific.

Ondrej Zenata, Zdenek Dvorak, Radim Vrzal

Reference Publications

Maszczyk, Mateusz et al.

Evaluation of Possible Neobavaisoflavone Chemosensitizing Properties towards Doxorubicin and Etoposide in SW1783 Anaplastic Astrocytoma Cells

Cells vol. 12,4 593.

DOI: 10.3390/cells12040593

Article Snippet: “Neomycin sulfate was acquired from Amara (Kraków, Poland). Annexin V binding buffer and Annexin V-CF488A conjugate were obtained from Biotium (Fremont, CA, USA).”

Jason W. Adams, Annabelle Vinokur, et al.

Loss of GTF2I promotes neuronal apoptosis and synaptic reduction in human cellular models of neurodevelopment

Cell Reports 43, 113867

DOI: https://doi.org/10.1016/j.celrep.2024.113867

Article Snippet: “Annexin V assay was performed⁶⁴^,⁶⁵ by first dissociating cortical organoids and monolayer neurons, which were subsequently resuspended in Annexin V binding buffer (Invitrogen) with Annexin V-CF488A conjugate (Biotium, Inc., Hayward, CA, USA) and Hoechst 33342 (Chemometec) and incubated for 15 min at 37°C.”

Vasty Osei-Amponsa, Monika Chandravanshi, et al.

hRpn13 shapes the proteome and transcriptome through epigenetic factors HDAC8, PADI4, and transcription factor NF-kB p50

Molecular Cell 84, 522–537

DOI: https://doi.org/10.1016/j.molcel.2023.11.035

Article Snippet: Highlight from the Critical commercial assays table “Biotium Mix-n-Stain Kit for CF488A Biotium Cat#92253, Biotium Mix-n-Stain Kit for CF568 Biotium Cat#92255, Annexin V – CF488A Conjugates Biotium Cat#29005.”

Beberok, A., Rok, J., Rzepka, Z. et al.

Interaction between moxifloxacin and Mcl-1 and MITF proteins: the effect on growth inhibition and apoptosis in MDA-MB-231 human triple-negative breast cancer cells

Pharmacol. Rep 74, 1025–1040 (2022).

DOI: https://doi.org/10.1007/s43440-022-00407-7

Article Snippet: “Annexin V-CF488A conjugate and Annexin V binding buffer were obtained from Biotium Inc. (USA). Antibodies: anti-Mcl-1 (4572, polyclonal antibody that detects an endogenous level of human Mcl-1, source: rabbit)”

Ondrej Zenata, Zdenek Dvorak, Radim Vrzal.

Pharmacologically relevant concentrations of berberine transiently stimulate dihydrotestosterone-inducible androgen receptor-mediated luciferase activity in human prostate cancer cells

Transl Cancer Res 2018;7(2):383-3

DOI: 10.21037/tcr.2018.03.31

Article Snippet: “Annexin V binding buffer and Annexin V-CF488A conjugate were from Biotium (Fremont, USA). LightCycler 480 Probes Master was from Roche Diagnostic Corporation”

Documents

SDS-Annexin-V-conjugates

CF® Annexin References

CF® Dyes Absorption and Emission Normalized Data

Cell Viability and Cell Death Brochure