No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

This kit contains NucView® 488 Caspase-3 Substrate to detect apoptotic cells and MitoView™ 633 mitochondrial membrane potential dye.

• Rapid, no-wash, endpoint or real-time assay
• Non-toxic, allowing multi-day experiments to be performed
• For flow cytometry, microscopy or live cell imaging systems
• Dual detection of caspase activity (green) and mitochondrial membrane potential (red/far-red)

NucView® 488 Caspase-3 Substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView™ 633 is a far-red fluorescent mitochondrial dye. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView™ 633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap. See a video of NucView® & MitoView™ in real-time imaging.

Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.

See our full selection of NucView® substrates and kits. For more information about NucView®, see the NucView flyer and reference list under the downloads tab, or see the NucView FAQs.