This kit contains CF®488A Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells for flow cytometry.
Product Description
This kit contains CF®488A Annexin V for staining apoptotic cells green, and 7-AAD for staining necrotic cells with far-red fluorescence, for detection by flow cytometry.
Features
- Simultaneous detection of apoptotic & necrotic cells
- 15-30 minute staining
- Next-generation CF®488A is brighter and more photostable than FITC
- For flow cytometry
Kit Components
- CF®488A Annexin V (human;recombinant, produced in E. coli)
- 7-AAD
- 5X Annexin Binding Buffer
Spectral Properties
- CF®488A Annexin V: Ex/Em 490/515 nm(FITC channel)
- 7-AAD: Ex/Em 543/655 nm (with DNA)(PE-Cy® or PerCP channel)
In apoptotic cells, the phospholipid phosphatidylserine (PS) is translocated from the inner to the outer surface of the plasma membrane, which targets the dying cells for phagocytosis. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid binding protein with a high affinity for PS. Annexin V labeled with CF®488A labels apoptotic cells with green fluorescence by binding to PS exposed on the outer leaflet. Our CF®488A dye is superior to fluorescein/FITC because it is not affected by pH and has far better photostability.
7-AAD is a membrane-impermeant nucleic acid dye that is excluded by live cells, but stains necrotic cells and late apoptotic cells. 7-AAD (Abs/Em with DNA = 543/655 nm) emits in the far-red spectrum and can be well separated from CF®488A and other green emitting dyes using the 488 nm line of a flow cytometer.
See our full selection of Cell Viability & Apoptosis Assays.
CF dye technology is covered by US and international patents. Cy dye is a registered trademark of GE Healthcare.
Reference Publications
Dmitriev, Petr et al.
Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis
Oncotarget vol. 7,40 (2016): 65090-65108
DOI: 10.18632/oncotarget.11368
Article Snippet: “Flow cytometry apoptosis assay was performed using a CF™488A Annexin V and 7-AAD Apoptosis Kit (Biotium, cat # 30060). 24 or 72 h after transfection, MB or BMSC cells were trypsinized, centrifuged, resuspended in PBS.”
Tsung-Hua Hsieh, Chia-Yi Hsu, et al.
Vorinostat decrease M2 macrophage polarization through ARID1A6488delG/HDAC6/IL-10 signaling pathway in endometriosis-associated ovarian carcinoma
Biomedicine & Pharmacotherapy 161 (2023) 114500
DOI: https://doi.org/10.1016/j.biopha.2023.114500
Article Snippet: “After 24 h of exposure to 10 nM vorinostat, the medium was removed, and the cells were fixed with 4% paraformaldehyde. The cells were stained with Annexin V and 7-AAD (#30060, Biotium, CA, USA), and the nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI).”
Rene Hernandez Delgadillo, Claudia María García-Cuéllar, et al.
In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells
International Journal of Nanomedicine 2018:13 6089–6097
DOI: http://dx.doi.org/10.2147/IJN.S179095
Article Snippet: “In order to analyze whether BisBAL NPs promote apoptosis among cancer cell lines, the CF®488A Annexin V and 7-AAD Apoptosis Kit (Biotium) was used according to the manufacturer’s instructions.”