PathoGreen™ Histofluorescent Stain is an anionic green fluorescent dye functionally similar to Fluoro-Jade dyes. These dyes stain degenerating neurons and their processes after exposure to a variety of neurotoxic insults in brain sections and cultured neurons.
Product Description
PathoGreen™ Histofluorescent Stain is an anionic green fluorescent dye (Ex/Em 497/520 nm) functionally similar to Fluoro-Jade® dyes. These dyes stain degenerating neurons and their processes after exposure to a variety of neurotoxic insults in brain sections and cultured neurons. The mechanism of neuronal staining by anionic fluorescent dyes has not been determined. It has been proposed that the negatively charged dyes bind to positively charged polyamines or other molecules specifically generated in dying neurons.
Fluoro-Jade is a registered trademark of Histo-Chem, Inc.
References
Shimada, R. et al. (2014). Neurological Research 36(4), 239-246. doi:10.1179/1743132813Y.0000000289
Product Attributes
Apoptosis/viability marker | Dead cell stain |
---|---|
For live or fixed cells | For fixed cells/tissue sections |
Detection method/readout | Fluorescence microscopy |
Assay type/options | Endpoint assay |
Colors | Green |
Excitation/Emission | 497/520 nm |
Reference Publications
Pedro Silva-Pinheiro, Raffaele Cerutti, Marta Luna-Sanchez, Massimo Zeviani, and Carlo Viscomi
A Single Intravenous Injection of AAV-PHP.B-hNDUFS4 Ameliorates the Phenotype of Ndufs4-/- Mice
Molecular therapy. Methods & clinical development vol. 17 1071-1078.
DOI: 10.1016/j.omtm.2020.04.026
Article Snippet: “Antibodies used included the following: anti-NDUFS4 (1:100 for immunohistochemistry [IHC] and 1:1,000 for western blot [WB]). PathoGreen histofluorescent stain (1,000X in water) was from Biotium (catalog no. 80027-5mL).”
Terjahna Richards, Jeanette C. Perron, Ketan Patel, John Wurpel, Sandra E. Reznik, and Francis Schanne
Therapeutic Intervention of Neuroinflammatory Alzheimer Disease Model by Inhibition of Classical Complement Pathway with the Use of Anti-C1r Loaded Exosomes
Research square rs.3.rs-3399248.
DOI: 10.21203/rs.3.rs-3399248/v1.
Article Snippet: “PathoGreen Histofluorescent stain (#80027–5) was purchased from Biotium (Fremont, CA, USA). Cy3-goat anti-rabbit IgG (#111–165-144) and Cy2-goat anti-mouse IgG (#115–225-146).”
Wiseman, Beth E
LC3-associated phagocytosis and its potential neuroinflammatory role in Parkinson’s Disease
the School of Biochemistry, Faculty of Life Sciences, University of Bristol
DOI: http://research-information.bristol.ac.uk
Article Snippet: Highlight from the Reagents table “1000X Pathogreen Histoflourescent stain Biotium 80027″