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Biowest DMEM Low Glucose w/ L-Glutamine w/ Sodium Pyruvate (L0060-500) Used For Scratch Wound Assay

Wound healing assays (Scratch wound assay) are widely utilized in cancer research and studies of cell-cell interactions to investigate cell migration, particularly in the context of skin wound repair and cancer metastasis. In this method, a confluent monolayer of cells is mechanically scratched to create a “wound,” and the rate of closure is monitored over time.

In the experiment, HaCaT were routinely cultured Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). All cells were cultured in a 37 degree Celsius, 5% carbon dioxide, humidified incubator. keratinocytes were routinely sub-cultured using 0.25 % trypsin and 1 mM Ethylenediaminetetraacetic acid. A linear scratch wound were created on a confluent layer of seeded cells and were treated in DMEM low glucose in the absence or presence of 10% FBS for 8 hours. Images were captured at regular intervals to analyse the wound closure over time.

Materials:

• Biowest Trypsin 0.25 % in PBS w/o Calcium w/o Magnesium w/o Phenol Red (Cat no: L0931-100 )
• Biowest DMEM Low Glucose w/ L-Glutamine w/ Sodium Pyruvate (Cat no: L0060-500)
• Biowest Fetal Bovine Serum (FBS) South America (Cat No: S1810-500)

Biowest DMEM Low Glucose w/ L-Glutamine w/ Sodium Pyruvate (L0060-500) Used For 3D In-vitro Skin

The EU has banned animal testing for cosmetics and their ingredients since 2013. A growing demand for in vitro skin models has been rising steadily to cater for both the safety testing (Skin irritation) and efficacy testing (functional test).

In the experiment, primary keratinocytes were seeded on a cultured insert to form a confluent layer. The confluent layer of cells is lifted to an air-liquid interface for 14 days to stimulates the keratinocytes to differentiate (stratification) and form a multi-layered epidermis that contains stratum corneum layers. The specialized medium primarily used consists of DMEM and Ham’s F12 in a specific ratio. It is chemically defined and serum-free to ensure a high reproducibility in result. Treatments can be topically applied to the skin model for a period of times. Histology such as H&E staining and other skin markers are commonly carried out. Gene expression and protein study are also alternative analysis method.

Materials:

• Biowest Trypsin 0.25 % in PBS w/o Calcium w/o Magnesium w/o Phenol Red ( Cat no: L0931-100 )
• Biowest DMEM Low Glucose w/ L-Glutamine w/ Sodium Pyruvate (Cat no: L0060-500)
• Biowest HAM’s F12 Glucose w/ L-Glutamine (Cat no: L0135-500)
• Atlantis Bioscience Keratinocyte Differentiation Media (Cat no: AS-9010)