Fetal Bovine Serum 500ml

Fetal Bovine Serum 500ml
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Fetal Bovine Serum 500ml

$497.00

$497.00

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Fetal bovine serum also commonly called fetal calf serum is the most widely used growth supplement for cell culture. It has high content growth factors and other nutrients. Choosing a high quality FBS is important for any researcher.

Biowest is the ideal partner for animal serum used for  translational research in various clinical, academic and industrial accounts across the world.

All our serum are sourced from BSE , FMD and BVD regions. Our very own Singapore Veterinary Authority has inspected all documentation and permits before the final product reaches your lab.

Cell Culture Tests:  Cell Growth, Plating Efficiency, Cloning Efficiency.

Cell Lines Tested

The following cell lines are tested with the serum:

  • HELA -Cancer Cell/Human.
  • L929 -Fibroblast-Mouse/ As Macrophage
  • SP2/0-AG14 -Mouse/Lymphoma
  • MRC- 5 -Human/Lung.

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  • Our standard grade, generally lower endotoxin than most brands(≤10 EU/mL endotoxin)
  • Lower haemoglobin levels than most brands(Hemoglobin, ≤25 mg/dL)
  • Sterile filtered; mycoplasma and virus tested
  • Proven success across wide spectrum of cell lines across several markets
  • Widely accepted in key universities like NUS, CSI ( Singapore) and various RND labs across SEA
  • All products are produced in ISO 9001:2008 and ISO 13485:2012 facilities.

 

Reference Publications

Konstantopoulos G, Leventakou D, Saltiel DR, Zervoudi E, Logotheti E, Pettas S, Karagianni K, Daiou A, Hatzistergos KE, Dafou D, Arsenakis M, Kottaridi C.

HPV16 E6 Oncogene Contributes to Cancer Immune Evasion by Regulating PD-L1 Expression through a miR-143/HIF-1a Pathway. Viruses.

Viruses vol. 16,1 113. 12 Jan. 2024

doi:10.3390/v16010113

Article Snippet: “SiHa, CaSki and HEK293T cell lines were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA) and grown in Dulbecco’s Modified Eagle medium (DMEM, Biowest #L0103) supplemented with 10% fetal bovine serum (FBS, Biowest #S1810) (Gibco/Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1% L-Glutamine (Biowest #X0550) and 1% Penicillin-Streptomycin (Biowest #L0022)…”

 

Mohamed, H. E., Asker, M. E., Kotb, N. S., & El Habab, A. M.

Human platelet lysate efficiency, stability, and optimal heparin concentration required in culture of mammalian cells

Blood research55(1), 35–43

DOI: https://doi.org/10.5045/br.2020.55.1.35

Article Snippet: “For HPL and FBS (#S1810-100, Biowest, Riverside, MO, USA) samples, the concentrations of IGF-1 and TGF-β1 were analyzed using enzyme-linked immunosorbent assay (ELISA) kits #IGF31-K01 (Eagle Biosciences, Inc., Amherst, NH, USA)”

 

Repossi R, Martín-Ramírez R, Gómez-Bernal F, Medina L, Fariña-Jerónimo H, González-Fernández R, Martín-Vasallo P, Plata-Bello J

Evaluation of Zonulin Expression and Its Potential Clinical Significance in Glioblastoma

Cancers. 2024; 16(2):356

DOI: https://doi.org/10.3390/cancers16020356

Article Snippet: “Dulbecco’s modified Eagle’s medium/Ham’s F-12 (DMEM/F12, Catalog No. 11330032; Gibco, Thermo Fisher Scientific, Waltham, MA, USA), containing L-Glutamine and 15 mM HEPES, supplemented with 10% fetal bovine serum (FBS, Catalog No. S1810; Biowest, Nuaillé, France), 59 mg/L Penicillin, 100 mg/L Streptomycin, and 25 mg/L Amphotericin (Catalog No. P3032, S9137 and A2411; Sigma-aldrich, San Luis, MO, USA)”

 

Lattmann, E., Lapaire, V., & Levesque, M. P.

Isolation and detection of extracellular vesicles from melanoma cells and liquid biopsies using size-exclusion chromatography and nano-flow cytometry

STAR protocols4(3), 102365

DOI: https://doi.org/10.1016/j.xpro.2023.102365

Article Snippet: “RPMI-1640 Sigma-Aldrich, USA R0883, Heat-inactivated fetal bovine serum (FBS) Biowest, France S1810, Exosome-depleted FBS, Gibco, USA A2720803, L-glutamine (200 mM) Gibco, USA 25030-24, Sodium pyruvate (100 mM) Sigma-Aldrich, USA S8636, Phosphate-buffered saline (PBS) Gibco, USA 14190144″
Dulbecco’s phosphate-buffered saline (DPBS) Gibco, USA 14190144
Sodium hydroxide (NaOH) pellets, 40 g/mol Merck, Germany 1310-73-2
PlasmoTest™ InvivoGen rep-pt1

 

Szymon Rutecki, Agnieszka Leśniewska-Bocianowska, Klaudia Chmielewska, Julia Matuszewska, Eryk Naumowicz, Paweł Uruski, Artur Radziemski, Justyna Mikuła-Pietrasik, Andrzej Tykarski, Krzysztof Książek

Serum starvation-based method of ovarian cancer cell dormancy induction and termination in vitro

Biology Methods and Protocols, Volume 8, Issue 1, 2023, bpad029

DOI: https://doi.org/10.1093/biomethods/bpad029

Article Snippet: “The A2780 and SKOV-3 cells were grown in RPMI-1640 medium (Biowest, Riverside, MO, USA, cat. no. L0501) with L-glutamine (2 mmol/l), penicillin (100 U/ml), streptomycin (100 g/ml) (Merck, cat. no. G6784), and 10% fetal bovine serum (FBS, Biowest, cat. no. S1810)

 

Zhao, X., Chen, M. & Tan, J.

Knockdown of ZFR suppresses cell proliferation and invasion of human pancreatic cancer

Biol Res 49, 26

DOI: https://doi.org/10.1186/s40659-016-0086-3

Article Snippet:Fetal bovine serum (FBS) was obtained from Biowest (Catalog Number: S1810, Loire Valley, France). The lentiviral expression vector (pFH-L) and packaging vectors (pVSVG-I and pCMVΔR8.92) were purchased from Hollybio (Shanghai, China)”

Documents

Blood Research

Cancers Article

Star Protocols

Oxford Biology

Biological Research

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