DMEM – F12 w/ L-Glutamine w/ 15mM Hepes 500ml

DMEM – F12 w/ L-Glutamine w/ 15mM Hepes 500ml

DMEM – F12 w/ L-Glutamine w/ 15mM Hepes 500ml



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Product Description

Classical Media

Many classical media are available with our partner Biowest portfolio. These include Dulbecco, Eagle, RPMI, Ham’s and many others. All of our products are suitable for cell culture applications and are made in modern ISO manufacturing facilities.

Product such as L0104 has proven to be compatible for cell lines  obtained from ATCC, DSMZ and Riken.


[accordion title=”key features”]

· Our most popular DMEM F-12 formulation

· DMEM F-12 w/ L-glutamine w/ 15mM Hepes

· Sterile filtered; 12 months shelf life

· Proven success across wide spectrum of cell lines across several markets

· Widely accepted in key institutions like NUS, CSI (Singapore) and various R&D laboratories across S.E.A.

· All products are produced in ISO 9001:2008 and ISO 13485:2012 facilities.

· ** please inquiry for powdered formulation


[accordion title=”Formulation”] l0093_formula.pdf[/accordion]
[accordion title=”MSDS/Tech Sheet”]l0093_msds.pdf


Reference Publications

Reference Publications

Arietta-García, A. G., Calzada, F., Ramírez-Sánchez, I., Bautista, E., García-Hernandez, N., & Ordoñez-Razo, R. M.

Advances in the Properties of Incomptine A: Cytotoxic Activity and Downregulation of Hexokinase II in Breast Cancer Cell Lines

International journal of molecular sciences24(15), 12406


Article Snippet: “Finally, MCF10A was maintained in DMEM/F12 medium (Biowest L0093-500) supplemented with 5% fetal horse serum (FHS) (GIBCO 16050130), 10 µg/mL of insulin, 0.5 µg/mL of hydrocortisone, 20 ng/mL of endothelial growth factor (EGF), and 1% antibiotic”


Akcay, G., & Luttge, R.

Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture

Micromachines12(2), 165


Article Snippet: “The SH-SY5Y cell line (94030304, Sigma Aldrich) was grown in T75 flasks Dulbecco’s Modified Eagle’s Medium: Nutrient Mixture F12 (DMEM/F12) (L0093, Biowest, Nuaille, France) supplemented with 10% Fetal Bovine Serum (FBS) (FBS; lot no. 11113, Bovogen, East Keilor [VIC], Australia)”


Jia Wen Kong,⊥ Zhiyong Lam,⊥ Kiat Hwa Chan, Rakesh Ganguly, Jia-Ying Joey Lee, Lit-Hsin Loo, Richard D. Webster, Zhen Xuan Wong, and Weng Kee Leong

Group VIII Metal Carbonyl Cluster-Boronic Acid Conjugates:
Cytotoxicity and Mode of Action Studies

ACS Omega 2021, 6, 29045−29053

DOI: 10.1021/acsomega.1c04116

Article Snippet: ” 1% penicillin/streptomycin (HyClone, UT). The MCF-10A cells were maintained in a DMEM-F12 medium (Biowest L0093, France) supplemented with 7.5% FBS (Gibco, NY), epidermal growth factor (Invitrogen), insulin (Sigma-Aldrich), hydrocortisone (Sigma-Aldrich) and
0.4% gentamicin (Gibco, NY), and 1% penicillin/streptomycin (HyClone, UT)”