DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate 500ml

DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate 500ml

DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate 500ml



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Classical Media

Many classical media are available with our partner Biowest portfolio. These include Dulbecco, Eagle, RPMI, Ham’s and many others. All of our products are suitable for cell culture applications and are made in modern ISO manufacturing facilities.

Product such as L0104 has proven to be compatible for cell lines obtained from ATCC, DSMZ and Riken.


[accordion title=”key features”]

· High Glucose w/ L-Glutamine w/o Sodium Pyruvate

· Sterile filtered; 12 months shelf life

· Proven success across wide spectrum of cell lines across several markets

· Widely accepted in key institutions like NUS, CSI (Singapore) and various R&D laboratories across S.E.A.

· All products are produced in ISO 9001:2008 and ISO 13485:2012 facilities.

· ** please inquiry for powdered formulation


[accordion title=”Formulation”] l0102_formula.pdf[/accordion]
[accordion title=”MSDS/Tech Sheet”]l0102_msds.pdf



Reference Publications

Ramirez-Sánchez DA, Navarro-Lleó N, Bäuerl C, Campista-León S, Coll-Marqués JM, Pérez-Martínez G.

Factors Affecting Spontaneous Endocytosis and Survival of Probiotic Lactobacilli in Human Intestinal Epithelial Cells

Microorganisms. 2022; 10(6):1142


Article Snippet: “IEC cultures of Caco-2 were maintained in DMEM (High Glucose, -Na-Pyruvate, Cat. No. L0102, Biowest, Riverside, MO, USA) supplemented with 10% heat inactivated fetal bovine serum (FBS), 1% non-essential amino acids (Cat. No. X0557, Biowest), 1% sodium pyruvate (NPY-B, Capricorn Scientific GmbH, Ebsdorfergrund, Germany), 1% L-glutamine (Cat. No. X0550, Biowest), 1% HEPES Buffer Solution (Cat. No. L0180, Biowest) and 1% penicillin-streptomycin (Cat. No. L0018-100, Biowest)”


Paula Montero, Inés Roger, Cristina Estornut, Javier Milara, Julio Cortijo,

Influence of dose and exposition time in the effectiveness of N-Acetyl-l-cysteine treatment in A549 human epithelial cells

Heliyon, Volume 9, Issue 5, 2023, e15613, ISSN 2405-8440,


Article Snippet: “Adenocarcinomic human bronchoalveolar basal epithelial cells (A549) were used to model ATII behavior [20]. A549 cells were purchased from ATCC (Cat.CCL-18 American Type Culture Collection; US) and cultured in high glucose DMEM (Cat. L0102, Biowest, US) supplemented with 10% FBS. N-Acetyl-l-cysteine (Cat.A7250) was purchased from Sigma-Aldrich”


Gielata, M., Karpińska, K., Gwiazdowska, A. et al.

Catulin reporter marks a heterogeneous population of invasive breast cancer cells with some demonstrating plasticity and participating in vascular mimicry

Sci Rep 12, 12673 (2022)


Article Snippet: “MDA-MB-231 and MCF7 cell lines were cultured in DMEM High Glucose (Biowest #L0102-500) supplemented with 10% FBS (Biowest, #S181S-500), 100 IU penicillin and 100 μg/mL streptomycin (Biowest #L0018-100). HCC1806 cells were cultured in RPMI-1640 (Biowest #L0500-500) supplemented with 10% FBS (Biowest, #S181S-500), 1 mM sodium pyruvate (Gibco), 100 IU penicillin and 100 μg/mL streptomycin (Biowest #L0018-100)”


AHN Changhwan, YOO Yeong-Min, PARK Mi-Jin, HAM Youngseok, YANG Jiyoon, JEUNG Eui-Bae

Cytotoxic Evaluation of the Essential Oils from Korean Native Plant on Human Skin and Lung Cells

J. Korean Wood Sci. Technol. 2021;49(4):371-383


Article Snippet:A549 (KCLB No: 10185) and Detroit 551 cells (KCLB No: 10110) from Korean cell line bank (Seoul, Republic of Korea) were seeded at a density of 0.5 × 10cells per well in 6-well plate and the medium used for cell culture was DMEM high glucose media (Catalog number: L0102, Biowest, Nuaillé, France) supplemented with penicillin-streptomycin solution (Catalog number: L0018 Biowest),”


Beyza Ecem Oz Bedir, Emine Terzi, Ozen Ozensoy Guler

Differential in vitro effects of oncogenic pathway inhibitors
on carbonic anhydrase-IX, xanthine oxidase, and catalase in
colorectal cancer

Int J Med Biochem 2023;6(1):36-41

DOI: 10.14744/ijmb.2022.50455

Article Snippet: “Human CRC cells HT29 were obtained from ATCC (HTB-38TM) and cultured in DMEM High Glucose Medium (Biowest, L0102) supplemented with 10% heat-inactivated FBS (Capricorn,FBS-HI-11A) at 37°C in a 5% CO2 humidified atmosphere.”


Adrianna Rutkowska, Tadeusz Strózik, Krystyna Jędrychowska-Dańska, Alicja Zamerska, Dorota Jesionek-Kupnicka, Tamara Kowalczyk, Waldemar Och, Błażej Szóstak, Cezary Tręda, Aneta Włodarczyk, Amelia Kierasińska-Kałka, Tomasz Wasiak, Damian Ciunowicz, Piotr Rieske, Ewelina Stoczyńska-Fidelus

Immunohistochemical detection of EGFRvIII in glioblastoma – Anti-EGFRvIII antibody validation for diagnostic and CAR-T purposes

Biochemical and Biophysical Research Communications, Volume 685, 2023, 149133, ISSN 0006-291X


Article Snippet: “For GMS10, the basal medium was DMEM with l-glutamine (Biowest, Cat. No. L0102). The Fibroblast Cell Line of the Human Foreskin (Cat. No. CRL-2522), U-87MG (Cat. No. HTB14), T98G (Cat. No. CRL-1690) and MDA-MB-468 (Cat. No. HTB-132) were purchased from the ATCC – American Type Culture Collection.”



Research Article

Scientific Reports

Wood Article

Medical Biochemistry