Hoechst 33258
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A red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected in flow cytometry and microscopy.
| SIZE |
CATALOG #
|
PRICE
|
| 10 mL | #40044 | $160 |
| 100 mL | #40045 | $151 |
PRODUCT ATTRIBUTES
| CAS number |
23491-45-4, 23491-52-3 |
|---|---|
| Cellular localization |
Nucleus |
| For live or fixed cells |
For fixed cells, For live/intact cells |
| Assay type/options |
DNA content/cell cycle (flow cytometry), Long term staining (24-72h), No-wash staining, Real-time imaging |
| Cell permeability |
Membrane permeant |
| Apoptosis/viability marker |
All cell stain |
| Fixation options |
Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde) |
| Colors |
Blue |
| Excitation/Emission |
350/461 nm (with DNA), 352/461 nm (with DNA) |
Hoechst Products
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Biotium offers Hoechst 33258 and Hoechst 33342 dyes in both solution or powder forms. Both dyes are spectrally similar but Hoechst 33528 is slightly more water soluble than Hoechst 33342. The dyes can be used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.
Hoechst can also be used to stain live bacteria (gram-positive and gram-negative), but in live yeast the staining is weak and not nuclear. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Hoechst 33258, pentahydrate
Hoechst 33342, trihydrochloride trihydrate
Learn more about Hoechst and our other novel nuclear stains. We also offer DAPI nuclear stains in powder or solution.
Having trouble with your experiment? See our section on troubleshooting tips for fluorescent staining.
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