AzureCyto High-Throughput In-Cell Western Kit

AzureCyto High-Throughput In-Cell Western Kit

High-Throughput Drug Screening Power: AzureCyto In-Cell Western Kit

 

Drug discovery researchers, accelerate your research with the AzureCyto In-Cell Western Kit! This all-in-one solution empowers you to perform high-throughput protein profiling, biomarker validation, and in situ analysis – all within a single, streamlined workflow.

Key Advantages for Drug Screening & Beyond:

  • Unmatched Throughput: Perform large-scale drug screens efficiently with a 96- or 384-well plate format, accelerating your path to promising drug candidates.
  • Simultaneous Biomarker Detection: Identify and validate potential disease, response, and treatment outcome markers with ease.
  • In Situ Analysis: Gain physiologically relevant insights by studying protein expression and activation within the complete cellular environment, eliminating the need for disruptive cell localization techniques.
  • Quantitative Measurements: Quantify protein levels, modifications, and signalling events with precision, unravelling the dynamics of cellular processes.

 

The AzureCyto In-Cell Western Kit Advantage:

  • Complete System for Accuracy: Eliminate variability with a pre-optimized kit containing all necessary reagents for staining whole cells and simultaneous detection of two biomarkers and total cell number for robust normalization.
  • Streamlined Workflow: Save time and resources with co-incubatable Total Cell Stain, minimizing assay steps.
  • Dual Biomarker Detection: Unveil complex signalling networks by detecting two distinct proteins simultaneously using our versatile AzureSpectra Secondary Antibodies.

 

Revolutionize your Drug Screening & Cellular Research with AzureCyto In-Cell Western Kit!

Order yours today and unlock the power of high-throughput, in situ protein analysis!

Technical Specifications

AzureCyto In-Cell Western Kit Components:

  • AzureCyto Permeabilization Solution: Ensures efficient antibody penetration for the detection of intracellular and nuclear proteins.
  • AzureCyto Block Solution: Specifically formulated for cell-based assays and fluorescence detection, minimizing background noise.
  • AzureCyto Total Cell Stain: Offers exceptional linearity and sensitivity for precise quantification across a broad range of cell volumes.
  • AzureSpectra Secondary Antibodies: Available in multiple spectral configurations for simultaneous detection of two biomarkers.

Technical Data

AzureCyto High-Throughput In-Cell Western Kit

1:2 serial dilution of HeLa cells seeded into a 96-well plate. (A) A composite image of three channels imaged simultaneously on the Sapphire™ FL at 100-micron resolution. (B) hnRNP K visualized with the 685 Standard Optical Module. (C) GAPDH visualized with the 784 Standard Optical Module. (D) AzureCyto stain visualized with the 532 Standard Optical Module. All images were taken on a Sapphire™ FL Biomolecular Scanner.

Linearity

AzureCyto Total Cell Stain is linear over a broad range of cell volumes.

Unlock the full spectrum of cellular protein expression with AzureCyto Total Cell Stain’s linear response, ensuring precise quantification and robust comparisons.

Sensitivity

AzureCyto Total Cell Stain makes normalization at low concentrations possible, allowing more meaningful comparisons of small amounts of protein and uncovering subtleties of cellular signalling.

Protocol

Protocol of AzureCyto In-Cell Western Kit

Short Protocol

AzureCyto In-cell Western Kit Short Protocol (Full Document)

Documents

Product Data Sheet

AzureCyto In-Cell Western Kit Product Data Sheet

 

Safety Data Sheet

AzureCyto Permeabilization Solution Safety Data Sheet
AzureCyto Blocking Buffer SDS

AzureCyto Total Cell Stain SDS

 

Product Flyer

Accurate and Reliable In-Cell Westerns with for Biomarker Discovery using AzureCyto

How In-Cell Westerns Can Advance Your Research