Radiance Q (150mL)

Radiance Q (150mL)


Chemiluminescent substrate for quantitative chemiluminescent Westerns

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

• Extended dynamic range for detecting low and high abundance proteins within the same blot
• Compare performance to SuperSignal West Dura and Amersham ECL Prime
• Sensitivity in mid femtograms
• Signal duration 10-24 hours

Product literature

Radiance Brochure

Which Substrate is Right for You?

Azure Biosystems Western Blotting Workflow

Application Note – Why You Should Leave the Darkroom

Application Note – Chemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your Experiment

Application Note – How to Improve Your Chemiluminescent Western Blots

Protocol and product insert

Radiance Q Short Protocol

Radiance Q Long Protocol

safety data sheets

Radiance SDS

Radiance and Radiance Plus Peroxide SDS

Reference Publications

Rohm M, Volke L, Schlaffke L, Rehmann R, Südkamp N, Roos A, Schänzer A, Hentschel A, Vorgerd M.

Dysregulation of Metabolism and Proteostasis in Skeletal Muscle of a Presymptomatic Pompe Mouse Model

Cells. 2023; 12(12):1602.

DOI: https://doi.org/10.3390/cells12121602

Article Snippet: “Antibody binding was detected by chemiluminescent substrate Radiance Q (AC2101, Azure Biosystems, Dublin, CA, USA) in the c600 Imaging System (Azure Biosystems, Dublin, CA, USA). The optical densities (OD) of the Coomassie staining were quantified using the Azure Biosystems software.”


Williams, C.H., Neitzel, L.R., Cornell, J. et al.

GPR68-ATF4 signaling is a novel prosurvival pathway in glioblastoma activated by acidic extracellular microenvironment

Exp Hematol Oncol 13, 13 (2024).

DOI: https://doi.org/10.1186/s40164-023-00468-1

Article Snippet: “The membranes were washed with PBST in four, five-minute intervals before protein visualization using Radiance Q (CAT # AC2101, Azure Biosystems, Dublin, CA, USA) on Bio-Rad ChemiDoc TM MP Imaging System (Hercules, CA, USA).”


Skowicki, Michal et al.

FAP Targeting of Photosensitizer-Loaded Polymersomes for Increased Light-Activated Cell Killing

Biomacromolecules vol. 25,2 (2024): 754-766.

DOI: 10.1021/acs.biomac.3c00943

Article Snippet:Membranes were washed 4 times with PBS-T, incubated for 30 s in Radiance Q-HRP chemiluminescence substrate (#AC2101, Azure), and imaged using a ChemiDoc MP imaging system.”


Korade, Zeljka et al.

Chronic Aripiprazole and Trazodone Polypharmacy Effects on Systemic and Brain Cholesterol Biosynthesis

Biomolecules vol. 13,9 1321.

DOI: 10.3390/biom13091321

Article Snippet:After three washes, the membranes were incubated with the secondary antibodies for 1 h at RT. After extensive washing, the blots were exposed to a chemiluminescence reagent (Radiance Q, Azure Biosystems AC2101) and visualized on an Azure 300 instrument (Azure Biosystems, Dublin, CA, USA).”


Catherine Rippe

Examining the Immune Regulation of NLRP12 Through Novel Protein Interactions

MSU Graduate Theses. 3837.

DOI: https://bearworks.missouristate.edu/theses/3837

Article Snippet: “The immunoblots were developed using chemiluminescence (Radiance Q, Cat. AC2101, Azure Biosystems) and visualized with an Azure C300 digital imager.”


Nguyen TGVargas-Blanco DA, Roberts LA, Shell SS.

The Impact of Leadered and Leaderless Gene Structures on Translation Efficiency, Transcript Stability, and Predicted Transcription Rates in Mycobacterium smegmatis

J Bacteriol 202:10.1128/jb.00746-19.

DOI: https://doi.org/10.1128/jb.00746-19

Article Snippet: “The membrane was rinsed as previously described and incubated with horseradish peroxidase (HRP) substrate (Radiance Q; Azure Biosystems; catalog number AC2101) as recommended by the manufacturer. Imaging was done using an Azure C200 imaging system (Azure Biosystems).”


Cells Article




Missouri State University

Research Article