Phosphatase Inhibitor Cocktail I (100× ddH2O)
Phosphatase Inhibitor Cocktail I (100× ddH2O)
Add to cart
It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein.
The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.
All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Composition
| Ingredient | CAS | MW | Concentration (100x) | Target | Type |
|---|---|---|---|---|---|
| Sodium Fluoride | 7681-49-4 | 41.99 | 100 mM | Acid Phosphatase | Reversible |
| Sodium Orthovanadate | 13721-39-6 | 183.91 | 100 mM | Alkaline phosphatase, PTPs, ATPases | Reversible |
| Sodium Molybdate | 7631-95-0 | 205.92 | 115 mM | Acid and phosphoprotein Phosphatase | Irreversible |
| Sodium Tartrate | 868-18-8 | 194.05 | 400mM | Acid Phosphatase | Reversible |
| Imidazole | 288-32-4 | 68.08 | 200 mM | Alkaline phosphatase | Reversible |
Features
- Accurate Ingredients —— Clearance of components and concentrations to avoid experimental uncertainty
- Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
- Cost-Effective —— Lower price with higher quality
Handling Instruction
- Compatible with Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, kinase assay and etc.
- Add concentrated cocktail at 1:100 (v/v) dilution to samples solution (such as cell lysates or tissue extracts) before assaying.
- Briefly vortex cocktails to help facilitate the dissolution.
Notice
- Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
- If Cocktail I and Cocktail II are used together, do not mix tube I with tube II beforehand, as there may be precipitation. To avoid this, please add them step by step during experiment.
HOW CAN WE HELP YOU? Our specialists are to help you find the best product for your application. We will be happy to help you find the right product for the job.
TALK TO A SPECIALIST
Contact our Customer Care, Sales & Scientific Assistance
EMAIL US
Consult and asked questions about our products & services
DOCUMENTATION
Documentation of Technical & Safety Data Sheet, Guides and more..
NEWSLETTER
We gladly support you by keeping you updated on our latest products and the developments around our services.
ATLANTIS BIOSCIENCE PTE LTD
362 Upper Paya Lebar Rd, #07-15,
Singapore 534963
WeChat ID: +65 8608 0974
© 2015-2023 Atlantis Bioscience Pte Ltd. All rights reserved. Co Reg No: 201539516N