PathoGreen™ Histofluorescent Stain, 1000X in water

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Biotium products are distributed only in Singapore and Thailand.

PathoGreen™ Histofluorescent Stain is an anionic green fluorescent dye functionally similar to Fluoro-Jade dyes. These dyes stain degenerating neurons and their processes after exposure to a variety of neurotoxic insults in brain sections and cultured neurons.

Product Description

PathoGreen™ Histofluorescent Stain is an anionic green fluorescent dye (Ex/Em 497/520 nm) functionally similar to Fluoro-Jade® dyes. These dyes stain degenerating neurons and their processes after exposure to a variety of neurotoxic insults in brain sections and cultured neurons. The mechanism of neuronal staining by anionic fluorescent dyes has not been determined. It has been proposed that the negatively charged dyes bind to positively charged polyamines or other molecules specifically generated in dying neurons.

Fluoro-Jade is a registered trademark of Histo-Chem, Inc.

References

Shimada, R. et al. (2014). Neurological Research 36(4), 239-246. doi:10.1179/1743132813Y.0000000289

Product Attributes

Apoptosis/viability marker	Dead cell stain
For live or fixed cells	For fixed cells/tissue sections
Detection method/readout	Fluorescence microscopy
Assay type/options	Endpoint assay
Colors	Green
Excitation/Emission	497/520 nm

Reference Publications

Pedro Silva-Pinheiro, Raffaele Cerutti, Marta Luna-Sanchez, Massimo Zeviani, and Carlo Viscomi

A Single Intravenous Injection of AAV-PHP.B-hNDUFS4 Ameliorates the Phenotype of Ndufs4-/- Mice

Molecular therapy. Methods & clinical development vol. 17 1071-1078.

DOI: 10.1016/j.omtm.2020.04.026

Article Snippet: “Antibodies used included the following: anti-NDUFS4 (1:100 for immunohistochemistry [IHC] and 1:1,000 for western blot [WB]). PathoGreen histofluorescent stain (1,000X in water) was from Biotium (catalog no. 80027-5mL).”

Terjahna Richards, Jeanette C. Perron, Ketan Patel, John Wurpel, Sandra E. Reznik, and Francis Schanne

Therapeutic Intervention of Neuroinflammatory Alzheimer Disease Model by Inhibition of Classical Complement Pathway with the Use of Anti-C1r Loaded Exosomes

Research square rs.3.rs-3399248.

DOI: 10.21203/rs.3.rs-3399248/v1.

Article Snippet: “PathoGreen Histofluorescent stain (#80027–5) was purchased from Biotium (Fremont, CA, USA). Cy3-goat anti-rabbit IgG (#111–165-144) and Cy2-goat anti-mouse IgG (#115–225-146).”

Wiseman, Beth E

LC3-associated phagocytosis and its potential neuroinflammatory role in Parkinson’s Disease

the School of Biochemistry, Faculty of Life Sciences, University of Bristol

DOI: http://research-information.bristol.ac.uk

Article Snippet: Highlight from the Reagents table “1000X Pathogreen Histoflourescent stain Biotium 80027″