Live-or-Dye? Fixable Viability Staining Kits

Live-or-Dye? Fixable Viability Staining Kits

Live-or-Dye? Fixable Viability Staining Kits

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Kits to covalently label dead cells, allowing cells with permeable plasma membranes to be excluded from analysis in flow cytometry. Includes dye options for fluorescence microscopy.

Product Description

Live-or-Dye™ Fixable Viability Staining Kits allow discrimination between live and dead cells by flow cytometry or microscopy. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.


  • Highly dead-cell specific
  • Covalent labeling with reactive dyes
  • Withstand fixation and permeabilization
  • The most available colors
  • Dye options designed for spectral flow

Kit Components

  • Live-or-Dye Fixable Dead Cell Dye
  • Anhydrous DMSO
    Note: Number of assays is based on 1 mL reaction size (106 cells); actual number of assays may vary depending on staining volume

Biotium offers a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis. Visit the Live-or-Dye™ Technology Page for more data and information.

How It Works

Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.

Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Special Application Notes

Live-or-Dye™ 510/550 & 665/685 have been validated for use in spectral flow cytometry on the Cytek® Aurora.

Live-or-Dye™ 488/515, 568/583, 594/614, & 640/662 have been validated in both flow cytometry and fluorescence microscopy.

Live-or-Dye™ 787/808 offers the best performance for the 808 laser line, effectively freeing up the channels in the visible spectrum.

Catalog No.

Viability Dye

Laser line

Emission filter

Abs/Em Max


32002, 32002-T Live-or-Dye™ 350/448 355 nm DAPI or Violet 347/448 nm Flow Cytometry
32003, 32003-T Live-or-Dye™ 405/452 405 nm Pacific Blue® 408/452 nm Flow Cytometry
32009, 32009-T Live-or-Dye™ 405/545 405 nm AmCyan 395/545 nm Flow Cytometry
32004, 32004-T Live-or-Dye™ 488/515 488 nm FITC 490/515 nm Flow Cytometry,
32012, 32012-T Live-or-Dye™ 510/550 488 nm Spectral scan 516/549 nm Spectral Cytometry
32005, 32005-T Live-or-Dye™ 568/583 488 or 561 nm PE 562/583 nm Flow Cytometry,
32006, 32006-T Live-or-Dye™ 594/614 488 or 561 nm PE-Texas Red® 593/614 nm Flow Cytometry,
32007, 32007-T Live-or-Dye™ 640/662 633 or 640 nm APC 642/662 nm Flow Cytometry, Microscopy
32013, 32013-T Live-or-Dye™ 665/685 633 or 640 nm Spectral scan 667/685 nm Spectral Cytometry
32008, 32008-T Live-or-Dye™ 750/777 633 or 640 nm APC-Cy®7 755/777 nm Flow Cytometry
32011, 32011-T Live-or-Dye™ 787/808 785 or 808 nm APC-Cy®7 or IR800 783/803 nm Flow Cytometry
32010, 32010-T Live-or-Dye NucFix™ Red 488 or 532 nm PE-Texas Red® 520/593 nm Flow Cytometry,


1. Methods (2015) 84;pp44-52 doi:10.1016/j.ymeth.2015.04.025 Live-or-Dye™ 488
2. Dev Comp Immunol (2017) Dec;77:95-105. doi: 10.1016/j.dci.2017.07.021 Live-or-Dye™ 350
3. PLoS ONE (2018) 13(12): e0208649 Live-or-Dye™ 640
3. Ann Surg Oncol (2018) 25, 3755–3763 doi:10.1245/s10434-018-6739-4 Live-or-Dye™ 750
4. MBio (2018) Aug 21;9(4) doi:10.1128/mBio.01403-18
5. Cell Physiol Biochem (2018) 49:828–836 doi:10.1159/000493110 Live-or-Dye™ 350
6. FEBS J (2019) Nov 26. doi:10.1111/febs.15156 Live-or-Dye™ 488
7. JBC (2019) 294, 16918-16929 doi:10.1074/jbc.RA119.009141 Live-or-Dye™ 488