ATP-Glo™ Bioluminometric Cell Viability Assay
ATP-Glo™ Bioluminometric Cell Viability Assay
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ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.
Product Description
ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay procedure involves a simply adding the ATP-Glo™ detection cocktail directly to cells cultured in complete culture medium. It is not necessary to remove medium or wash cells before adding the reagent.
Features:
- Highly sensitive, detect from a single cell to tens of thousands of cells
- Excellent linearity over 6 orders of magnitude
- Homogeneous, no-wash assay
- Detect ATP amount or quantify number of live cells
Kit Components:
- D-Luciferin
- Firefly luciferase (recombinant, produced in E. coli)
- ATP-Glo™ assay buffer
- 2 mM ATP standard
This ATP detection kit takes advantage of firefly luciferase’s use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available. Because ATP is an indicator of metabolically active cells, the number of viable cells can be assessed based on the amount of ATP present.
The ATP-Glo™ kit can be used to detect as little as a single cell or 0.01 picomole of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity.
Please note:
ATP-Glo™ is a flash-type luminescence assay. The luminescence signal generated is stable for up to 1 minute. This assay is designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format.
More Luciferase Kits
| Catalog number | Product | Description |
|---|---|---|
| 30085 | Firefly Luciferase Assay Kit 2.0 | Flash-type firefly luciferase assay kit. |
| 30075 | Firefly Luciferase Assay Kit (Lyophilized) | Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C. |
| 99923 | 5X Firefly Lysis Buffer | Extra lysis buffer for use with Firefly Luciferase Assay Kits, only required if using well sizes larger than 24-well. |
| 30028 | Steady-Luc™ Firefly HTS Assay Kit | Glow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates. |
| 30028L | Steady-Luc Firefly HTS Assay Kit (Lyophilized) | Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C. |
| 30082 | Renilla Luciferase Assay Kit 2.0 | Flash-type Renilla luciferase assay kit. |
| 99912 | 5X Passive Lysis Buffer | Extra lysis buffer for use with Renilla Luciferase Assay Kits, only required if using well sizes larger than 24-well. |
| 30081 | Firefly and Renilla Luciferase Single Tube Assay Kit | Assay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube. |
| 99821-100mL | 1X Passive Lysis Buffer 2.0 | Extra lysis buffer for use with Firefly & Renilla Single Tube Assay Kits, only required if using well sizes larger than 24-well. |
| 30020 | ATP-Glo™ Bioluminometric Cell Viability Assay Kit | Flash-type luminescence-based assay for measuring cell viability or ATP concentration. |
| 22003 | Mini Cell Scrapers | Mini-cell scrapers, useful for lysing cells in 96-well, 48-well, or 24-well plates. |
References
1. PNAS (2008) 105(32), 11218-11223. https://doi.org/10.1073/pnas.0801661105
2. Biochim Biophys Acta. (2009) 1790(3), 208–212. doi:10.1016/j.bbagen.2008.12.005
3. J Biol Chem (2012) 287(47), 39776–39788. DOI 10.1074/jbc.M112.382986
4. Dalton Trans (2014) 43, 8395-8404. https://doi.org/10.1039/C4DT00024B
5. J Biol Chem (2014) 289(23), 16278–16289. DOI 10.1074/jbc.M114.559914
6. Oncotarget (2015) 6(16),14233-46. https://doi.org/10.18632/oncotarget.3899
7. Oncotarget (2016) 7(7), 8399-412. doi: 10.18632/oncotarget.6724
8. BBA – Mol Basis Disease (2017) 1863, 2171–2181. https://doi.org/10.1016/j.bbadis.2017.06.004
9. Cell Death Disease (2017) 8, e3081. doi:10.1038/cddis.2017.453
10. Mol Neurobiol (2017) 54, 6107–6119. https://doi.org/10.1007/s12035-016-0145-3
11. Front Oncol (2018) 8, 552. doi: 10.3389/fonc.2018.00552
12. Int J Biol Sci (2018) 14(13), 1873-1882. doi: 10.7150/ijbs.27746
13. J Cell Mol Med (2018) 22, 5720–5731. DOI: 10.1111/jcmm.13848
14. Mol Neurobiol (2018) 55, 9139–9155. https://doi.org/10.1007/s12035-018-1062-4
15. Mol Cell Neurosci (2019) 96, 1–9. https://doi.org/10.1016/j.mcn.2019.01.003
Product Attributes
| Apoptosis/viability marker | Metabolic activity |
|---|---|
| For live or fixed cells | Cell lysis required |
| Assay type/options | Luminescence (flash-type), Endpoint assay, Homogeneous assay |
| Detection method/readout | Luminometer (single-tube or microplate reader with reagent injectors) |
| Product origin | Firefly luciferase; recombinant, produced in E. coli |
| Storage Conditions | Store at -60 °C or below |
Protocols
SDS
Supporting Documents
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