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ACE2 Mouse Models – Humanized and Conditional/Knockout Mice

Mouse models play a critical role in both vaccine and drug development. Studies have shown that SARS-CoV-2 binds to the angiotensin-converting enzyme 2 (ACE2) receptor on the surface of human cells through the spike protein (S protein).
Many different hACE2 transgenic mouse models have been developed for in vivo experimental platform for COVID-19 research. We are prioritizing our support in COVID-19 research. Check out the list of ACE2 mouse models in the table below.

ACE2 Mouse Models

ACE2 Mouse Models	Background Strain	Description
Humanized ACE2 (hACE2) Mice (All Humanisation)	C57BL/6JGpt	· CRISPR/Cas9 Knockin (KI) · Mouse ACE2 replaced by human ACE2 · Human ACE2 expressed under the control of mouse promoter
Humanized ACE2 (hACE2) Mice (All Humanisation)	BALB/cJGpt
Humanized ACE2 (hACE2) Mice (Extracellular Humanisation)	C57BL/6JGpt	· CRISPR/Cas9 Knockin (KI) · Mouse-derived ACE2 signal peptide inserted into the human-mouse chimeric CDS · Human-mouse chimeric ACE2 expressed under the control of mouse promoter
NCG-hACE2	NOD/ShiLtJGpt	· CRISPR/Cas9 Knockin (KI) · Triple-immunodeficient mice · Mouse ACE2 replaced by human ACE2 · Human ACE2 expressed under the control of mouse promoter · Can be immuno-humanized using PBMC or CD34+ cell engraftments
H11-K18-hACE2	C57BL/6JGpt	· CRISPR/Cas9 Knockin (KI) · Mouse model that over-expresses human ACE2 under K18 (human cytokeratin 18) promoter at the safe island H11 site
ACE2 Conditional Knockout Model	C57BL/6JGpt	· Conditional knockout (cKO) CRISPR/Cas9 · ACE2 knockout in specific tissues and cells after mating with mice expressing Cre recombinase
ACE2 Knockout Model	C57BL/6JGpt	· CRISPR/Cas9 Knockout (KO) · ACE2 global knockout

Selected Publications:

Ma X, Zou F, Yu F, et al., Nanoparticle vaccines based on the receptor binding domain (RBD) and heptad repeat (HR) of SARS-CoV-2 elicit robust protective immune responses. Immunity 2020 53(6):1315-1330. e9; doi.org/10.1016/j.immuni.2020.11.015 (IF: 31.745)
Qiao J, Li Y-S, Zeng R, et al., SARS-CoV-2 M pro inhibitors with antiviral activity in a transgenic mouse model. Science 2021;371(6536):1374-1378; DOI: 10.1126/science.abf1611 (IF 47.728)
Li T, Han X, Gu C, et al., Potent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants. Nature communications 2021;12(1):1-11; doi.org/10.1038/s41467-021-26539-7 (IF: 14.919)
Zhang S, Huang W, Ren L, et al., Comparison of viral RNA–host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Cell Research 2021:1-15 doi.org/10.1038/s41422-021-00581-y (IF:25.617)

Custom Animal Models for COVID-19 Research

We also offer custom animal models to meet the requirements for your COVID-19 study. If you need other COVID-19 models, send in your inquiries and we will assist you.

Documents

hACE2 Extracellular humanization strain info

H11-K18-hACE2 strain info

B6-HuACE2_FL strain info

NCG-hACE2(all humanization) strain info

BALB_c-hACE2(all humanization) strain info

Reference Publications

Brodaczewska, K.K., Bielecka, Z.F., Maliszewska‑Olejniczak, K., Szczylik, C., Porta, C., Bartnik, E., & Czarnecka, A.M.

Metastatic renal cell carcinoma cells growing in 3D on poly‑D‑lysine or laminin present a stem‑like phenotype and drug resistance.

Oncology Reports, 42, 1878-1892. (2019).

DOI: https://doi.org/10.3892/or.2019.7321

Article Snippet: “…and then detached with Accutase (cat. no. L0950; Biowest) to obtain single-cell suspensions, washed 2 times with serum-free and antibiotic-free DMEM (product code, L0501; Biowest) to remove remaining FBS and used in experiments.”

Paolo Manzini, Valeria Peli, Araceli Rivera-Ordaz, Silvia Budelli, Mario Barilani, Lorenza Lazzari

Validation of an automated cell counting method for cGMP manufacturing of human induced pluripotent stem cells

Biotechnology Reports, Volume 33 ,2022, e00708, ISSN 2215-017X,
DOI: https://doi.org/10.1016/j.btre.2022.e00708.

Article Snippet: “To obtain single cell suspensions, hiPSC were incubated with accutase (L0950; Biowest, Nuaillé, France) for 5 min at 37 °C and collected in d-PBS without Ca²⁺ and Mg²⁺ (ECB4004L; Euroclone, Pero, Italy).”

Ramirez Ortega, D., Ovalle Rodríguez, P., Pineda, B. et al.

Kynurenine Pathway as a New Target of Cognitive Impairment Induced by Lead Toxicity During the Lactation.

Sci Rep 10, 3184 (2020).

DOI: https://doi.org/10.1038/s41598-020-60159-3

Article Snippet: “Mice brains were washed with cold PBS 1Χ and cut into small pieces, then 1 ml of accutase (cat. No. L0950, BioWest) was added.”

Cuenca-Ortolá I, Martínez-Rojas B, Moreno-Manzano V, García Castelló M, Monleón Pradas M, Martínez-Ramos C, Más Estellés J.

A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes.

Biomedicines. 2022; 10(11):2736.

DOI: https://doi.org/10.3390/biomedicines10112736

Article Snippet: “Neurospheres were dissociated before seeding using Accutase (L0950-100, Biowest) following the manufacturer’s instructions. Dissociated NPCs were seeded in 2 drops (6 × 10⁵ cells/3 µL drop) to promote their dispersion around the PLA membrane.”

Reeh, H., Rudolph, N., Billing, U. et al.

Response to IL-6 trans- and IL-6 classic signalling is determined by the ratio of the IL-6 receptor α to gp130 expression: fusing experimental insights and dynamic modelling.

Cell Commun Signal 17, 46 (2019)

DOI: https://doi.org/10.1186/s12964-019-0356-0

Article Snippet: “After stimulation with IL-6 or Hy-IL-6 as indicated, HepG2 and HepG2-IL-6Rα cells were detached from cell culture dishes with 1 ml Accutase (Biowest, Nuaillé, France, Cat. No. L0950–100).”

Maxime RF Gosselin, Virginie Mournetas, Malgorzata Borczyk, Suraj Verma, Annalisa Occhipinti, Justyna Róg, Lukasz Bozycki, Michal Korostynski, Samuel C Robson, Claudio Angione, Christian Pinset, Dariusz C Gorecki

Loss of full-length dystrophin expression results in major cell-autonomous abnormalities in proliferating myoblasts

eLife 11:e75521. (2022)

DOI: https://doi.org/10.7554/eLife.75521

Article Snippet: “Cells were detached from dishes using Accutase (Biowest L0950). ”