Dissociation reagents

Name: Accutase - 100ml
SKU: L0950-100
Availability: InStock

Atlantis is proud to partner with Biowest to provide high-quality dissociation products for the use of tissues and monolayer cells. We aim to provide the right research tool for you the researcher. Do send us an email at [email protected] if there is something you need.

Accutase is a ready-to-use cell detachment solution which combines proteolytic and collagenolytic activities allowing a broad range of applications from cell detachment to tissue dissociation. It is alternative to trypsin for the detachment and dissociation of anchorage-dependent cells from surfaces. It can also be used on suspension cells to reduce clumping in preparation for counting. A variety of cells, like hESCs, tumor cell lines, keratinocytes, fibroblasts, endothelial cells and primary cells, have been proven to detach effectively with Accutase.

Which dissociation agent is suitable for you?

	Fast ;Effective	Gentle & Effective	Specialized for pharma/vaccine
Cat no	X0930-100	L0950-100	L0941-100
Animal origin	YES	NO	NO
High Cell Viablity	YES	YES	YES
Inactivation method by inhibitors	YES	NO	YES
Package size	100ml	100ml	100ml

Key Benefits:

No neutralization required: Streamline your process with a time-saving solution.
Preserves cell viability: Maintains cell health and function after detachment.
Wide range of applications: Suitable for various cell types and experimental setups.
Proven performance: Effective on fibroblasts, keratinocytes, endothelial cells, and many more.
High quality: Manufactured in ISO 9001:2008 and ISO 13485:2012 certified facilities.

Experience the Accutase difference in your research.

We deliver Biowest solutions across South East Asia (Singapore, Thailand, Vietnam, Indonesia, Philippines, Myanmar) and Across China Only.

Reference Publications

Brodaczewska, K.K., Bielecka, Z.F., Maliszewska‑Olejniczak, K., Szczylik, C., Porta, C., Bartnik, E., & Czarnecka, A.M.

Metastatic renal cell carcinoma cells growing in 3D on poly‑D‑lysine or laminin present a stem‑like phenotype and drug resistance.

Oncology Reports, 42, 1878-1892. (2019).

DOI: https://doi.org/10.3892/or.2019.7321

Article Snippet: “…and then detached with Accutase (cat. no. L0950; Biowest) to obtain single-cell suspensions, washed 2 times with serum-free and antibiotic-free DMEM (product code, L0501; Biowest) to remove remaining FBS and used in experiments.”

Paolo Manzini, Valeria Peli, Araceli Rivera-Ordaz, Silvia Budelli, Mario Barilani, Lorenza Lazzari

Validation of an automated cell counting method for cGMP manufacturing of human induced pluripotent stem cells

Biotechnology Reports, Volume 33 ,2022, e00708, ISSN 2215-017X,
DOI: https://doi.org/10.1016/j.btre.2022.e00708.

Article Snippet: “To obtain single cell suspensions, hiPSC were incubated with accutase (L0950; Biowest, Nuaillé, France) for 5 min at 37 °C and collected in d-PBS without Ca²⁺ and Mg²⁺ (ECB4004L; Euroclone, Pero, Italy).”

Ramirez Ortega, D., Ovalle Rodríguez, P., Pineda, B. et al.

Kynurenine Pathway as a New Target of Cognitive Impairment Induced by Lead Toxicity During the Lactation.

Sci Rep 10, 3184 (2020).

DOI: https://doi.org/10.1038/s41598-020-60159-3

Article Snippet: “Mice brains were washed with cold PBS 1Χ and cut into small pieces, then 1 ml of accutase (cat. No. L0950, BioWest) was added.”

Cuenca-Ortolá I, Martínez-Rojas B, Moreno-Manzano V, García Castelló M, Monleón Pradas M, Martínez-Ramos C, Más Estellés J.

A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes.

Biomedicines. 2022; 10(11):2736.

DOI: https://doi.org/10.3390/biomedicines10112736

Article Snippet: “Neurospheres were dissociated before seeding using Accutase (L0950-100, Biowest) following the manufacturer’s instructions. Dissociated NPCs were seeded in 2 drops (6 × 10⁵ cells/3 µL drop) to promote their dispersion around the PLA membrane.”

Reeh, H., Rudolph, N., Billing, U. et al.

Response to IL-6 trans- and IL-6 classic signalling is determined by the ratio of the IL-6 receptor α to gp130 expression: fusing experimental insights and dynamic modelling.

Cell Commun Signal 17, 46 (2019)

DOI: https://doi.org/10.1186/s12964-019-0356-0

Article Snippet: “After stimulation with IL-6 or Hy-IL-6 as indicated, HepG2 and HepG2-IL-6Rα cells were detached from cell culture dishes with 1 ml Accutase (Biowest, Nuaillé, France, Cat. No. L0950–100).”

Maxime RF Gosselin, Virginie Mournetas, Malgorzata Borczyk, Suraj Verma, Annalisa Occhipinti, Justyna Róg, Lukasz Bozycki, Michal Korostynski, Samuel C Robson, Claudio Angione, Christian Pinset, Dariusz C Gorecki

Loss of full-length dystrophin expression results in major cell-autonomous abnormalities in proliferating myoblasts

eLife 11:e75521. (2022)

DOI: https://doi.org/10.7554/eLife.75521

Article Snippet: “Cells were detached from dishes using Accutase (Biowest L0950). “