Human Interleukin 1 Beta (IL1b) ELISA Kit

Human IL1b (Interleukin 1 Beta) sandwich ELISA Kit specific for IL1b in cosmetic and personal care products research & development.

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Overview of Human IL1b (Interleukin 1 Beta)

Product name:	Human IL1b(Interleukin 1 Beta) ELISA Kit
Reactivity:	Human
Alternative Names:	IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
Assay Type:	Sandwich
Sensitivity:	5.8 pg/mL
Standard:	1000 pg/mL
Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine; Tumour immunity; Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta(IL1b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Beta(IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Interleukin 1 Beta(IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Beta(IL1b) in the samples is then determined by comparing the OD of the samples to the standard curve.

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[accordion title= “Standard Curve“]

Concentration (ng/mL)	OD	Corrected OD
1000.00	2.207	2.125
500.00	1.613	1.531
250.00	1.115	1.033
125.00	0.757	0.675
62.50	0.421	0.339
31.25	0.297	0.215
15.63	0.195	0.113
0.00	0.082	0.000

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[accordion title= “Precision“]

Intra-assay Precision (Precision within an assay)： CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)： CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. [/accordion] [accordion title= "Recovery“]

Matrices listed below were spiked with a certain level of recombinant IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL1b in samples.

Matrix	Recovery range	Average
serum(n=5)	92-105%	98%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	79-93%	86%

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[accordion title=”Linearity“]

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.