Human HCAM(Homing Associated Cell Adhesion Molecule) ELISA Kit
Human HCAM(Homing Associated Cell Adhesion Molecule) ELISA Kit
Human HCAM(Homing Associated Cell Adhesion Molecule) sandwich ELISA Kit is sensitive and selective for the detection of HCAM.
Add to cart
Overview of Human HCAM(Homing Associated Cell Adhesion Molecule) ELISA Kit
| Product name: | Human HCAM(Homing Associated Cell Adhesion Molecule) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | CDW44; CD44; CSPG8; ECMR-III; HCELL; IN; LHR; MC56; MDU2; MDU3; MIC4; MUTCH-I; Pgp1; Hematopoietic Cell E- And L-Selectin Ligand; Chondroitin Sulfate Proteoglycan 8 |
| Assay Type: | Sandwich |
| Sensitivity: | 0.56 ng/mL |
| Standard: | 100 ng/mL |
| Range: | 1.57-100 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Signal transduction; CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule; Hematology; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homing Associated Cell Adhesion Molecule(HCAM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Homing Associated Cell Adhesion Molecule(HCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Homing Associated Cell Adhesion Molecule(HCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a color change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Homing Associated Cell Adhesion Molecule(HCAM) in the samples is then determined by comparing the OD of the samples to the standard curve. |
[accordions]
[accordion title= “Standard Curve“]
| Concentration (ng/mL) | OD | Corrected OD |
| 100.00 | 2.054 | 1.958 |
| 50.00 | 1.630 | 1.534 |
| 25.00 | 1.096 | 1.000 |
| 12.50 | 0.861 | 0.765 |
| 6.25 | 0.593 | 0.497 |
| 3.13 | 0.305 | 0.209 |
| 1.57 | 0.215 | 0.119 |
| 0.00 | 0.096 | 0.000 |
[/accordion]
[accordion title= “Precision“]
Intra-assay Precision (Precision within an assay)： CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)： CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. [/accordion] [accordion title= "Recovery“]
Matrices listed below were spiked with certain levels of recombinant HCAM and the recovery rates were calculated by comparing the measured value to the expected amount of HCAM in samples.
| Matrix | Recovery range | Average |
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 78-93% | 85% |
| Heparin plasma(n=5) | 78-95% | 86% |
[/accordion]
[accordion title=”Linearity“]
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HCAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-101% | 84-101% | 95-104% | 88-105% |
| EDTA plasma(n=5) | 93-102% | 87-96% | 85-98% | 91-103% |
| Heparin plasma(n=5) | 92-102% | 81-93% | 86-98% | 91-103% |
[/accordion]
HOW CAN WE HELP YOU? Our specialists are to help you find the best product for your application. We will be happy to help you find the right product for the job.
TALK TO A SPECIALIST
Contact our Customer Care, Sales & Scientific Assistance
EMAIL US
Consult and asked questions about our products & services
DOCUMENTATION
Documentation of Technical & Safety Data Sheet, Guides and more..
NEWSLETTER
We gladly support you by keeping you updated on our latest products and the developments around our services.
ATLANTIS BIOSCIENCE PTE LTD
362 Upper Paya Lebar Rd, #07-15,
Singapore 534963
WeChat ID: +65 8608 0974
© 2015-2023 Atlantis Bioscience Pte Ltd. All rights reserved. Co Reg No: 201539516N