Human NOS2/iNOS (NitricOxide Synthase 2, Inducible) ELISA Kit
Human NOS2/iNOS (NitricOxide Synthase 2, Inducible) ELISA Kit
Human NOS2/iNOS(NitricOxide Synthase 2, Inducible) sandwich ELISA Kit specific to Nitric Oxide Synthase 2, Inducible for cosmetic research.
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Overview of Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
| Product name: | Human NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible |
| Assay Type: | Sandwich |
| Sensitivity: | 0.054 ng/mL |
| Standard: | 10 ng/mL |
| Range: | 0.16-10 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Enzyme & Kinase; Metabolic pathway; Tumor immunity; Cardiovascular biology; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NitricOxide Synthase 2, Inducible(NOS2/iNOS). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NitricOxide Synthase 2, Inducible(NOS2/iNOS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain NitricOxide Synthase 2, Inducible(NOS2/iNOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of nitric oxide Synthase 2, Inducible(NOS2/iNOS) in the samples is then determined by comparing the OD of the samples to the standard curve. |
[accordions]
[accordion title= “Standard Curve“]
| Concentration (ng/mL) | OD | Corrected OD |
| 10.00 | 1.984 | 1.899 |
| 5.00 | 1.586 | 1.501 |
| 2.50 | 1.174 | 1.089 |
| 1.25 | 0.797 | 0.712 |
| 0.63 | 0.529 | 0.444 |
| 0.32 | 0.347 | 0.262 |
| 0.16 | 0.187 | 0.102 |
| 0.00 | 0.085 | 0.000 |
[/accordion]
[accordion title= “Precision“]
Intra-assay Precision (Precision within an assay)：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. [/accordion] [accordion title= "Recovery“]
Matrices listed below were spiked with certain level of recombinant NOS2/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2/iNOS in samples.
| Matrix | Recovery range | Average |
| serum(n=5) | 95-107% | 101% |
| EDTA plasma(n=5) | 85-95% | 90% |
| Heparin plasma(n=5) | 79-93% | 86% |
[/accordion]
[accordion title=”Linearity“]
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-96% | 86-98% | 91-103% | 87-90% |
| EDTA plasma(n=5) | 81-94% | 85-97% | 93-102% | 81-95% |
| Heparin plasma(n=5) | 83-98% | 92-103% | 86-97% | 93-101% |
[/accordion]
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