Human ET-1(Endothelin1) ELISA Kit for Cosmetic & Personal Care Research

Human ET-1(Endothelin1) Sandwich ELISA Kit for cosmetic and personal care products research can be applied to Endocrinology and Cardiovascular biology research areas.

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Overview of Human ET-1 (Endothelin1) ELISA Kit for Cometic & Personal Care Research

Product name:	Human ET-1(Endothelin1) ELISA Kit
Reactivity:	Human
Alternative Names:	ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
Assay Type:	Sandwich
Sensitivity:	2.71 pg/mL
Standard:	500 pg/mL
Range:	7.82-500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelin1(ET-1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Endothelin1(ET-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelin1(ET-1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelin1(ET-1) in the samples is then determined by comparing the OD of the samples to the standard curve.

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[accordion title= “Standard Curve“]

Concentration (ng/mL)	OD	Corrected OD
500.00	2.057	1.951
250.00	1.498	1.392
125.00	0.983	0.877
62.50	0.755	0.649
31.25	0.507	0.401
15.63	0.329	0.223
7.82	0.208	0.102
0.00	0.106	0.000

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[accordion title= “Precision“]

Intra-assay Precision (Precision within an assay)：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. [/accordion] [accordion title= "Recovery“]

Matrices listed below were spiked with certain level of recombinant ET-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ET-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	90-105%	97%

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[accordion title=”Linearity“]

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ET-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.