Ethidium Homodimer III (EthD-III)

A red fluorescent dead cell stain for bacteria and mammalian cells

SIZE	CATALOG #	PRICE
1 mL	#40050	$250

PRODUCT ATTRIBUTES

Cellular localization	Nucleus & cytoplasm
Assay type/options	Real-time imaging
Cell permeability	Membrane impermeant
Apoptosis/viability marker	Dead cell stain
Colors	Red
Excitation/Emission	279, 522/593 nm (with DNA)

PRODUCT DESCRIPTION

Reference Publications

Yan, Wei-Tao et al.

PANoptosis-like cell death in ischemia/reperfusion injury of retinal neurons

Neural regeneration research vol. 18,2 (2023): 357-363.

DOI: 10.4103/1673-5374.346545

Article Snippet: “Ethidium Homodimer III (EthD-III; Biotium, Fremont, CA, USA, Cat# 40050) staining combined with DSF was used to indicate pyroptosis. DSF inhibits GSDMD expression and interferes with pore formation triggered by GSDMD in the cell membrane to prevent the release of interleukin and other inflammatory factors (Zhang et al., 2021a, b).”

Hsieh, Huai-Ching et al.

Characterization and identification of cell death dynamics by quantitative phase imaging

Journal of biomedical optics vol. 27,4 (2022): 046502.

DOI: 10.1117/1.JBO.27.4.046502

Article Snippet: “After treating the cells for 1.5 h, we stained the cells by CellEvent Caspase-3/7 Green Detection Reagent (Caspase-3/7, ThermoFisher Scientific) and Ethidium Homodimer III (EthD-III, Biotium), a DNA dye for dead cells only.”

Dong, Y., Huang, Y., Zhang, Z. et al.

iRGD-modified memory-like NK cells exhibit potent responses to hepatocellular carcinoma

J Transl Med 21, 205 (2023).

DOI: https://doi.org/10.1186/s12967-023-04024-7

Article Snippet: “After incubation, MCSs were treated with Calcein AM and Ethidium homodimer III (EthD-III) solutions (Viability/Cytotoxicity Assay Kit, biotium, USA) as per the manufacturer’s instructions.”

Tyszka-Czochara M, Konieczny P, Majka M.

Caffeic Acid Expands Anti-Tumor Effect of Metformin in Human Metastatic Cervical Carcinoma HTB-34 Cells: Implications of AMPK Activation and Impairment of Fatty Acids De Novo Biosynthesis

International Journal of Molecular Sciences. 2017; 18(2):462.

DOI: https://doi.org/10.3390/ijms18020462

Article Snippet: “Fluorescent dyes Annexin-V (excitation/emission 490/515 nm) and Ethidium homodimer (EthD-III, excitation/emission 528/617 nm) were used (Biotium, Fremont, CA, USA). The cells were gated according to forward (FSC), side scatter (SSC), and appropriate fluorescence parameters.”

Maeda, E., Ando, Y., Takeshita, K. et al.

Through the cleared aorta: three-dimensional characterization of mechanical behaviors of rat thoracic aorta under intraluminal pressurization using optical clearing method

Sci Rep 12, 8632 (2022).

DOI: https://doi.org/10.1038/s41598-022-12429-5

Article Snippet: “The specimen was incubated in 5 µM ethidium homodimer-III (Biotium, USA) in PBS overnight at 4 °C to stain the nuclei of smooth muscle cells and was imaged under a two-photon microscope (A1R MP, Nikon, Japan) in a noncleared, normal state within PBS.”