Ethidium Homodimer III (EthD-III), 1 mM in DMSO

Ethidium Homodimer III (EthD-III), 1 mM in DMSO

Ethidium Homodimer III (EthD-III), 1 mM in DMSO

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Biotium products are distributed only in Singapore and Thailand.

A red fluorescent dead cell stain for bacteria and mammalian cells

SIZE
CATALOG #
 
PRICE
 
200 uL #40051

$250

PRODUCT ATTRIBUTES

Cellular localization

Nucleus & cytoplasm

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Colors

Red

Excitation/Emission

279, 522/593 nm (with DNA)

PRODUCT DESCRIPTION

Ethidium Homodimer III, also known as EthD-III, is a red fluorescent dead cell stain for bacteria and mammalian cells. It is a cell membrane-impermeant nucleic acid dye that stains only dead cells with damaged cell membranes. Ethidium Homodimer III was developed by Biotium as a superior alternative to Ethidium Homodimer I. The absorption and emission spectra are similar, but EthD-III is 45% brighter.

Ethidium Homodimer III is membrane impermeant and useful for selectively staining dead cells with damaged plasma membranes. It also is a component of several combination viability assay kits for detecting both live and dead cells in the same population, such as our Viability/Cytotoxicity Assay Kit for Animal Cells (Cat. No 30002) and our Viability/Cytotoxicity Assay Kit for Bacteria (Cat. No. 30027).

We also offer Ethidium Homodimer III in solid form (catalog no. 40050).

EthD-III is dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Product Features:

  • λEx/λEm (with DNA) = 522/593 nm* (EthD-III also has a large absorption peak at 279 nm)
  • 1 mM solution in DMSO
  • Store at 4°C and protect from light
  • MW: ~1000

 

Documents

Protocols

PI-40050 40051

 

SDS

sds-40051

 

Supporting Documents

Cell Viability and Cell Death, 

Cellular stains,

Nuclear stains