7-AAD

7-AAD

7-AAD

Add to cart
Description

Product Description

A red fluorescent dead cell stain for bacteria and mammalian cells

SIZE
CATALOG #
 
PRICE
 
1 mg #40037

$116

1 mL(1mg/mL) #40084

$155

PRODUCT ATTRIBUTES

Cellular localization

Nucleus

Assay type/options

DNA content/cell cycle (flow cytometry)

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Colors

Far-red

Excitation/Emission

546/647 nm (with DNA)

PRODUCT DESCRIPTION
7-AAD products
Product Catalog Number Unit Size Format
7-AAD 40037 1 mg Orange/red solid
7-AAD Solution, 1 mg/mL 40084 1 mL Red solution in DMSO:Water (1:1)

7-AAD is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.  The dye is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity.

7-AAD/DNA complexes can be excited at 488 nm with an argon-ion laser, and has a large Stokes shift with an emission maxima of 647 nm (1-3). 7-AAD intercalates selectively at GC-rich regions of DNA, which makes the dye useful for chromosome banding studies (4). 7-AAD can be used in multicolor fluorescence microscopy and flow cytometry.

We offer 7-AAD as a solid powder (catalog no. 40037) or as a solution in 1 mg/mL DMSO:Water (1:1) (catalog no. 40084).

Product Features:

  • λExEm (with DNA) = 546/647 nm (7-AAD can also be excited by the 488 or 532 laser lines)
  • Orange red solid soluble in DMF or DMSO
  • Store at -20°C and protect from light, especially in solution
  • C62H87N13O16
  • MW: 1270.45

We also sell a kit containing 7-AAD in conjunction with our fluorescently labeled Annexin V. The CF®488A Annexin V and 7-AAD Apoptosis kit contains CF®488A Annexin V for staining apoptotic cells green, and 7-AAD for staining necrotic cells with far-red fluorescence.

Ethidium homodimer III and propidium iodide (PI) are alternative dead-cell selective stains. Also see our Live-or-Dye™ Fixable Viability Staining Kits.

[accordions]

Reference Publications

Reference Publications

Lu H-I, Chen K-L, Yen C-Y, Chen C-Y, et al.

Michelia compressa-Derived Santamarine Inhibits Oral Cancer Cell Proliferation via Oxidative Stress-Mediated Apoptosis and DNA Damage

Pharmaceuticals. 2024; 17(2):230.

DOI: https://doi.org/10.3390/ph17020230

Article Snippet:A 75% ethanol solution was applied for cell fixation. Following overnight cooling, cells were washed with PBS. Then, 1 μg/mL 7AAD (Biotium, Inc., Hayward, CA, USA) was added to the cell suspension for 30 min”

 

Yu T-J, Shiau J-P, Tang J-Y, Farooqi AA, Cheng Y-B, Hou M-F, Yen C-H, Chang H-W.

Physapruin A Exerts Endoplasmic Reticulum Stress to Trigger Breast Cancer Cell Apoptosis via Oxidative Stress

International Journal of Molecular Sciences. 2023; 24(10):8853.

DOI: https://doi.org/10.3390/ijms24108853

Article Snippet:Cells were harvested by 0.05% Trypsin-EDTA. 5 μg/mL of 7-aminoactinmycin D (7AAD) (Biotium, Hayward, CA, USA), a DNA dye, was used to stain the DNA content of 70% ethanol-fixed cells for 30 min”

 

Shiau J-P, Lee M-Y, Tang J-Y, Huang H, Lin Z-Y, Su J-H, Hou M-F, Cheng Y-B, Chang H-W.

Marine Sponge Aaptos suberitoides Extract Improves Antiproliferation and Apoptosis of Breast Cancer Cells without Cytotoxicity to Normal Cells In Vitro

Pharmaceuticals. 2022; 15(12):1575.

DOI: https://doi.org/10.3390/ph15121575

Article Snippet:Fixed cells were incubated with 1 μg/mL of 7AAD (Biotium; Hayward, CA, USA). The DNA content of 7AAD-positive cells was then detected by the Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).”

 

Chen Y-N, Chan C-K, Yen C-Y, et al.

Antioral Cancer Effects by the Nitrated [6,6,6]Tricycles Compound (SK1) In Vitro

Antioxidants. 2022; 11(10):2072.

DOI: https://doi.org/10.3390/antiox11102072

Article Snippet:Cells were stained with 7-aminoactinmycin D (7AAD; 1 μg/mL; 30 min) (Biotium; Hayward, CA, USA) for routine flow cytometry (Accuri C6, Becton-Dickinson, Mansfield, MA, USA). The subG1 population was individually counted.”