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Ethidium Homodimer III (EthD-III), 1 mM in DMSO- Categories:
Apoptosis Assays, Cell Viability and Apoptosis
$0.00 -
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Ethidium Homodimer III (EthD-III), 1 mM in DMSO
- Categories:
Apoptosis Assays, Cell Viability and Apoptosis
$0.00 -
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Ethidium Homodimer I (EthD-I)
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Biotium products are distributed only in Singapore and Thailand.
Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a mixed live-dead population and also used as a counterstain in multicolor fluorescent assays.
| SIZE |
CATALOG #
Â
|
PRICE
Â
|
| 1 mL | #40010 |
$183 |
|
0.5 mL (2 mM in DMSO) |
#40044 |
$198 |
PRODUCT ATTRIBUTES
| CAS number |
61926-22-5 |
|---|---|
| DNA/RNA dye |
DNA & RNA dye |
| Cellular localization |
Nucleus & cytoplasm |
| Cell permeability |
Membrane impermeant |
| Detection method/readout |
Fluorescence microscopy, Flow cytometry |
| Apoptosis/viability marker |
Dead cell stain |
| Colors |
Red |
| Excitation/Emission |
528/617 nm (with DNA) |
| Molecular weight |
857 |
PRODUCT DESCRIPTION
Ethidium Homodimer IÂ dye (EthD-I) is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution or for selectively staining dead cells with damaged plasma membranes in all cell types, including mammalian cells, bacteria and yeast.
- High-affinity red fluorescent nucleic acid stain
- Dead cell specific in all cell types, including mammalian cells, bacteria and yeast
- Available as a high-purity powder or solution
Â
| Product | Catalog Number | Unit Size | Format |
|---|---|---|---|
| Ethidium Homodimer (solid) | 40010 | 1 mg | Red solid |
| Ethidium Homodimer (2 mM in DMSO) | 40014 | 0.5 mL | Red solution |
Ethidium Homodimer I is offered at high purity grade that is not available from other manufacturers. Ethidium Homodimer from other suppliers often contains a high amount (as much as 20%) of inorganic salt that lowers the weight percent purity. The dye is not base pair specific and is believed to label nucleic acid by intercalation.
Find the Right Stain for Your Application
EthD-I binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. EthD-1 is a widely used alternative to Propidium Iodide since the high affinity of EthD-I permits the use of a lower dye concentration and no-wash staining. Biotium developed Ethidium Homodimer III, as a superior alternative to EthD-I. The absorption and emission spectra are similar, but EthD-III is 45% brighter. Biotium also offers other dead-cell stains in other colors and with other beneficial properties such as Live-or-Dye NucFix™ Red which, unlike EthD-I, is a fixable dead-cell stain. See our Cellular Stains Selection Guide and Cellular Stains Table for more information. Learn more about EthD-I and other dead-cell specific stains.
Biotium also offers other live-cell specific stains in other colors and with other beneficial properties. Such as NucSpot® Live Cell Nuclear Stains, which are cell-membrane permeable DNA dyes that specifically stain nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have low toxicity for live cell imaging.
Reference Publications
Leung, Ada W Y et al. Combined Use of Gene Expression Modeling and siRNA Screening Identifies Genes and Pathways Which Enhance the Activity of Cisplatin When Added at No Effect Levels to Non-Small Cell Lung Cancer Cells In Vitro PloS one vol. 11,3 e0150675. DOI: 10.1371/journal.pone.0150675 Article Snippet: “At 72 hours post-treatment, the cells were stained for 20 minutes with dye exclusion marker Hoechst 33342 (16.2 μM, Invitrogen, Carlsbad, CA) and the nuclear stain ethidium homodimer I (EthD-I; 1μM, Biotium, Hayward, CA) and then imaged via the IN Cell Analyzer 1000″  Scialis, Renato J, and José E Manautou. Elucidation of the Mechanisms through Which the Reactive Metabolite Diclofenac Acyl Glucuronide Can Mediate Toxicity The Journal of pharmacology and experimental therapeutics vol. 357,1 (2016): 167-76. DOI: 10.1124/jpet.115.230755 Article Snippet: “Ethidium homodimer-1 was purchased from Biotium Inc. (Hayward, CA). All LC–MS/MS solvents were of high analytical grade and were purchased from Burdick and Jackson (Muskegon, MI).”  Tao Jiang, et al. Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics Biofabrication 12 015024 DOI: 10.1088/1758-5090/ab3a5c Article Snippet: “MCTSs viability was determined using calcein-AM (AAT Bioquest, Inc) and ethidium homodimer-1 (EthD-1, Biotium) assay. Calcein-AM is a cell-permeant component that enters the cells and is cleaved by esterases inside the living cells, producing an intense green fluorescence.”
