10X Fish Gelatin Blocking Agent

10X Fish Gelatin Blocking Agent is a concentrated solution of gelatin purified from cold-water fish skin, designed to minimize non-specific antibody binding in immunodetection procedures such as Western blotting, immunofluorescence (IF), and immunohistochemistry (IHC).

Fish gelatin effectively blocks non-specific binding sites on positively charged nylon or PVDF membranes and can also be used for antibody blocking and dilution during immunostaining. Unlike BSA or milk-based blockers, fish gelatin contains no IgG or serum proteins, eliminating the risk of cross-reactivity with mammalian antibodies and improving signal specificity.

Supplied as a convenient 10X stock solution, this product simplifies buffer preparation while ensuring consistent blocking performance across experiments.

Features

• Gelatin purified from cold-water fish skin
• Reduces non-specific antibody binding
• Free of IgG and serum proteins
• No cross-reactivity with mammalian antibodies
• Suitable for Western blot, IF, and IHC
• Convenient 10X concentrated solution

Applications

• Western blot membrane blocking (PVDF or nylon)
• Immunofluorescence (IF) staining
• Immunohistochemistry (IHC)
• Antibody blocking and dilution in immunoassays

*Biotium products are only available in Singapore and Thailand.