Reference Publications

Lu H-I, Chen K-L, Yen C-Y, Chen C-Y, et al. Michelia compressa-Derived Santamarine Inhibits Oral Cancer Cell Proliferation via Oxidative Stress-Mediated Apoptosis and DNA Damage Pharmaceuticals. 2024; 17(2):230. DOI: https://doi.org/10.3390/ph17020230 Article Snippet: “A 75% ethanol solution was applied for cell fixation. Following overnight cooling, cells were washed with PBS. Then, 1 μg/mL 7AAD (Biotium, Inc., Hayward, CA, USA) was added to the cell suspension for 30 min”   Yu T-J, Shiau J-P, Tang J-Y, Farooqi AA, Cheng Y-B, Hou M-F, Yen C-H, Chang H-W. Physapruin A Exerts Endoplasmic Reticulum Stress to Trigger Breast Cancer Cell Apoptosis via Oxidative Stress International Journal of Molecular Sciences. 2023; 24(10):8853. DOI: https://doi.org/10.3390/ijms24108853 Article Snippet: “Cells were harvested by 0.05% Trypsin-EDTA. 5 μg/mL of 7-aminoactinmycin D (7AAD) (Biotium, Hayward, CA, USA), a DNA dye, was used to stain the DNA content of 70% ethanol-fixed cells for 30 min”   Shiau J-P, Lee M-Y, Tang J-Y, Huang H, Lin Z-Y, Su J-H, Hou M-F, Cheng Y-B, Chang H-W. Marine Sponge Aaptos suberitoides Extract Improves Antiproliferation and Apoptosis of Breast Cancer Cells without Cytotoxicity to Normal Cells In Vitro Pharmaceuticals. 2022; 15(12):1575. DOI: https://doi.org/10.3390/ph15121575 Article Snippet: “Fixed cells were incubated with 1 μg/mL of 7AAD (Biotium; Hayward, CA, USA). The DNA content of 7AAD-positive cells was then detected by the Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).”   Chen Y-N, Chan C-K, Yen C-Y, et al. Antioral Cancer Effects by the Nitrated [6,6,6]Tricycles Compound (SK1) In Vitro Antioxidants. 2022; 11(10):2072. DOI: https://doi.org/10.3390/antiox11102072 Article Snippet: “Cells were stained with 7-aminoactinmycin D (7AAD; 1 μg/mL; 30 min) (Biotium; Hayward, CA, USA) for routine flow cytometry (Accuri C6, Becton-Dickinson, Mansfield, MA, USA). The subG1 population was individually counted.”