Reference Publications

Yosta de Stigter, Harmen J. van der Veer, Bas J. H. M. Rosier, and Maarten Merkx Bioluminescent Intercalating Dyes for Ratiometric Nucleic Acid Detection ACS Chem. Biol. 2024, 19, 2, 575–583 DOI: https://doi.org/10.1021/acschembio.3c00755 Article Snippet: “An amine-reactive N-hydroxysuccinimide (NHS) ester of Thiazole Orange (TO) was obtained from Biotium (cat. no. 40073),Both components were dissolved in DMSO and added into a 100 μL-reaction containing 24 mM (1 equiv) of TO, 20 mM (0.8 equiv) 1,2-(aminoethyl)maleimide cross-linker, and 72 mM of DIPEA (3 equiv).”   Eman Amer Husein Alwattar kinetic and mechanistic studies of fading of fluorescent dyes and application in vital fields School of Chemistry Cardiff University. DOI: orca.cardiff.ac.uk Article Snippet: “Thiazole orange occurs predominantly as a monomer in aqueous medium, where its fluorescence is small due to intramolecular movements. At low concentrations, TO forms H-dimer and H-aggregates”