Anti-tumor activity of BET inhibitors in androgen-receptorexpressing triple-negative breast cancer

Anti-tumor activity of BET inhibitors in androgen-receptorexpressing triple-negative breast cancer

Anti-tumor activity of BET inhibitors in androgen-receptorexpressing triple-negative breast cancer

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Abstract

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Triple-negative breast cancer (TNBC) is a heterogeneous disease comprising several subtypes. Androgen-receptor (AR) signaling has been targeted by several investigational agents in luminal AR subtype TNBCs. Bromodomain (BRD) and extra-terminal motif (BET) protein inhibitors have been shown to attenuate AR signaling in metastatic castration-resistant prostate cancer and to overcome enzalutamide resistance. We demonstrated potent anti-tumor efects of the BET inhibitor JQ1 against AR-positive TNBC cell lines using cell viability and cell cycle analysis. To reveal the mechanisms of JQ1 efects, multiplex gene expression analysis and immunoblotting assays were used. We examined in vivo efects of JQ1 in a xenograft model of AR expressing TNBC. JQ1 exhibited its anti-proliferative activity by inducing apoptosis and cell cycle arrest. JQ1 activity was not mediated by MYC downregulation. Instead, JQ1 blocked the interactions among the ATPase-family AAA-domain-containing 2 protein (ATAD2), BRD2, BRD4, and AR; efectively suppressing the expression of AR associated targets. In addition, JQ1 showed signifcant anti-tumor activity in vivo in TNBC xenograft mouse models as a monotherapy and in combination with anti-AR therapy. Taken together, our results showed that the BET inhibitor JQ1 is a promising therapeutic agent for the treatment of AR-positive TNBC.

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In this study, MDA-MB-231 and MDA-MB-453 cells in charcoal-stripped serum media were used to evaluate drug effects on DHT-mediated proliferation. Learn more about Biowest’s standard fetal bovine serum and speciality fetal bovine serum.

DOI:

doi:10.1038/s41598-019-49366-9[/vc_column_text][/vc_column][/vc_row][vc_row][vc_column][vc_column_text css=”.vc_custom_1598017023330{margin-bottom: 0px !important;}”]

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