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TrueBlack® Lipofuscin Autofluorescence Quencher is the preferred quencher for detection of mRNA in human neuronal populations

After testing a panel of lipofuscin quenching methods, including quenching with cupric sulfate, bleaching in Dent’s fixative, detergent extraction, Murray’s clearing, treatment with 8% SDS, and standard Sudan Black blocking, Biotium’s TrueBlack® Lipofuscin Autofluorescence Quencher was determined to be the only acceptable option for overcoming lipofuscin autofluorescence without causing V3HCR signal loss.

Massively multiplexed nucleic acid detection with Cas13

Cheri M. Ackerman, Cameron Myhrvold, Sri Gowtham Thakku, Catherine A. Freije, Hayden C. Metsky, David K. Yang, Simon H. Ye, Chloe K. Boehm, Tinna-Sólveig F. Kosoko-Thoroddsen, Jared Kehe, Tien G. Nguyen, Amber Carter, Anthony Kulesa, John R. Barnes, Vivien G. Dugan, Deborah T. Hung, Paul C. Blainey & Pardis C. Sabeti.Massively multiplexed nucleic acid detection with Cas13.Nature. 2020 Jun;582(7811):277-282. doi: 10.1038/s41586-020-2279-8

A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation

Gürhan Özkayar, Ege Mutlu, Şebnem Şahin, Yağmur Demircan Demircan Yalçın, Taylan Töral, Haluk Külah, Ender Yıldırım, Özge Zorlu, Ebru Özgür.A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation.Micromachines (Basel). 2020 Oct 30;11(11):981. doi: 10.3390/mi11110981

Engineering of Primary Pancreatic Islet Cell Spheroids for Three-dimensional Culture or Transplantation: A Methodological Comparative Study

Wassmer CH, Bellofatto K, Perez L, Lavallard V, Cottet-Dumoulin D, Ljubicic S, Parnaud G, Bosco D, Berishvili E, Lebreton F. Engineering of Primary Pancreatic Islet Cell Spheroids for Three-dimensional Culture or Transplantation: A Methodological Comparative Study. Cell Transplant. 2020 Jan-Dec;29:963689720937292. doi: 10.1177/0963689720937292. Erratum in: Cell Transplant. 2020 Jan-Dec;29:963689720969271. PMID: 32749168; PMCID: PMC7563811.

Allosteric SHP2 Inhibitor, IACS-13909, Overcomes EGFR-Dependent and EGFR-Independent Resistance Mechanisms toward Osimertinib

Yuting Sun, Brooke A. Meyers, Barbara Czako, Paul Leonard, Faika Mseeh, Angela L. Harris, Qi Wu, Sarah Johnson, Connor A. Parker, Jason B. Cross, Maria Emilia Di Francesco, Benjamin J. Bivona, et. al. ,Allosteric SHP2 Inhibitor, IACS-13909, Overcomes EGFR-Dependent and EGFR-Independent Resistance Mechanisms toward Osimertinib. Cancer Res. 2020 Nov 1;80(21):4840-4853. doi: 10.1158/0008-5472.CAN-20-1634. Epub 2020 Sep 14.

Preclinical models of nicotinamide phosphoribosyltransferase inhibitor-mediated hematotoxicity and mitigation by co-treatment with nicotinic acid

Jacqueline M Tarrant 1, Preeti Dhawan, Jatinder Singh, Tanja S Zabka, Emer Clarke, Garry DosSantos, Peter S Dragovich, Deepak Sampath, Tori Lin, Bobbi McCray, Nghi La, Trung Nguyen, Ariel Kauss, Donna Dambach, Dinah L Misner, Dolores Diaz, Hirdesh Uppal.Preclinical models of nicotinamide phosphoribosyltransferase inhibitor-mediated hematotoxicity and mitigation by co-treatment with nicotinic acid.Toxicol Mech Methods. 2015 Mar;25(3):201-11. doi: 10.3109/15376516.2015.1014080.

Regulation of the RNAPII Pool Is Integral to the DNA Damage Response

Tufegdžić Vidaković, A., Mitter, R., Kelly, G. P., Neumann, M., Harreman, M., Rodríguez-Martínez, M., Herlihy, A., Weems, J. C., Boeing, S., Encheva, V., Gaul, L., Milligan, L., Tollervey, D., Conaway, R. C., Conaway, J. W., Snijders, A. P., Stewart, A., & Svejstrup, J. Q. (2020). Regulation of the RNAPII Pool Is Integral to the DNA Damage Response. Cell, 180(6), 1245–1261.e21. https://doi.org/10.1016/j.cell.2020.02.009I2

Quantitative analysis of taxane drug target engagement of microtubules in circulating tumor cells from metastatic castration resistant prostate cancer patients treated with CRXL301, a nanoparticle of docetaxel

Mukhtar, E., Worroll, D., Galletti, G., Schuster, S., Piha-Paul, S. A., &Giannakakou, P. (2020). Quantitative analysis of taxane drug target engagement of microtubules in circulating tumor cells from metastatic castration resistant prostate cancer patients treated with CRXL301, a nanoparticle of docetaxel. Cancer Drug Resistance.https://cdrjournal.com/article/view/3425

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