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TrueBlack® Lipofuscin Autofluorescence Quencher is the preferred quencher for detection of mRNA in human neuronal populations

After testing a panel of lipofuscin quenching methods, including quenching with cupric sulfate, bleaching in Dent’s fixative, detergent extraction, Murray’s clearing, treatment with 8% SDS, and standard Sudan Black blocking, Biotium’s TrueBlack® Lipofuscin Autofluorescence Quencher was determined to be the only acceptable option for overcoming lipofuscin autofluorescence without causing V3HCR signal loss.

Massively multiplexed nucleic acid detection with Cas13

Cheri M. Ackerman, Cameron Myhrvold, Sri Gowtham Thakku, Catherine A. Freije, Hayden C. Metsky, David K. Yang, Simon H. Ye, Chloe K. Boehm, Tinna-Sólveig F. Kosoko-Thoroddsen, Jared Kehe, Tien G. Nguyen, Amber Carter, Anthony Kulesa, John R. Barnes, Vivien G. Dugan, Deborah T. Hung, Paul C. Blainey & Pardis C. Sabeti.Massively multiplexed nucleic acid detection with Cas13.Nature. 2020 Jun;582(7811):277-282. doi: 10.1038/s41586-020-2279-8

A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation

Gürhan Özkayar, Ege Mutlu, Şebnem Şahin, Yağmur Demircan Demircan Yalçın, Taylan Töral, Haluk Külah, Ender Yıldırım, Özge Zorlu, Ebru Özgür.A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation.Micromachines (Basel). 2020 Oct 30;11(11):981. doi: 10.3390/mi11110981

Engineering of Primary Pancreatic Islet Cell Spheroids for Three-dimensional Culture or Transplantation: A Methodological Comparative Study

Wassmer CH, Bellofatto K, Perez L, Lavallard V, Cottet-Dumoulin D, Ljubicic S, Parnaud G, Bosco D, Berishvili E, Lebreton F. Engineering of Primary Pancreatic Islet Cell Spheroids for Three-dimensional Culture or Transplantation: A Methodological Comparative Study. Cell Transplant. 2020 Jan-Dec;29:963689720937292. doi: 10.1177/0963689720937292. Erratum in: Cell Transplant. 2020 Jan-Dec;29:963689720969271. PMID: 32749168; PMCID: PMC7563811.

Allosteric SHP2 Inhibitor, IACS-13909, Overcomes EGFR-Dependent and EGFR-Independent Resistance Mechanisms toward Osimertinib

Yuting Sun, Brooke A. Meyers, Barbara Czako, Paul Leonard, Faika Mseeh, Angela L. Harris, Qi Wu, Sarah Johnson, Connor A. Parker, Jason B. Cross, Maria Emilia Di Francesco, Benjamin J. Bivona, et. al. ,Allosteric SHP2 Inhibitor, IACS-13909, Overcomes EGFR-Dependent and EGFR-Independent Resistance Mechanisms toward Osimertinib. Cancer Res. 2020 Nov 1;80(21):4840-4853. doi: 10.1158/0008-5472.CAN-20-1634. Epub 2020 Sep 14.

AG-221, a First-in-Class Therapy Targeting Acute Myeloid Leukemia Harboring Oncogenic IDH2 Mutations

Kenichi Iwai, Tadahiro Nambu, Ryo Dairiki, Momoko Ohori, Jie Yu, Kristine Burke, Masamitsu Gotou, Yukiko Yamamoto, Shunsuke Ebara, Sachio Shibata, Ryosuke Hibino, Satoru Nishizawa, Tohru Miyazaki, Misaki Homma, Yuya Oguro, Takashi Imada, Nobuo Cho, Noriko Uchiyama, Akifumi Kogame, Toshiyuki Takeuchi, Osamu Kurasawa, Kazunori Yamanaka, Huifeng Niu, Akihiro Ohashi. Molecular mechanism and potential target indication of TAK-931, a novel CDC7-selective inhibitor.Sci Adv. 2019 May 22;5(5):eaav3660. doi: 10.1126/sciadv.aav3660.

Preclinical models of nicotinamide phosphoribosyltransferase inhibitor-mediated hematotoxicity and mitigation by co-treatment with nicotinic acid

Jacqueline M Tarrant 1, Preeti Dhawan, Jatinder Singh, Tanja S Zabka, Emer Clarke, Garry DosSantos, Peter S Dragovich, Deepak Sampath, Tori Lin, Bobbi McCray, Nghi La, Trung Nguyen, Ariel Kauss, Donna Dambach, Dinah L Misner, Dolores Diaz, Hirdesh Uppal.Preclinical models of nicotinamide phosphoribosyltransferase inhibitor-mediated hematotoxicity and mitigation by co-treatment with nicotinic acid.Toxicol Mech Methods. 2015 Mar;25(3):201-11. doi: 10.3109/15376516.2015.1014080.

Regulation of the RNAPII Pool Is Integral to the DNA Damage Response

Tufegdžić Vidaković, A., Mitter, R., Kelly, G. P., Neumann, M., Harreman, M., Rodríguez-Martínez, M., Herlihy, A., Weems, J. C., Boeing, S., Encheva, V., Gaul, L., Milligan, L., Tollervey, D., Conaway, R. C., Conaway, J. W., Snijders, A. P., Stewart, A., & Svejstrup, J. Q. (2020). Regulation of the RNAPII Pool Is Integral to the DNA Damage Response. Cell, 180(6), 1245–1261.e21. https://doi.org/10.1016/j.cell.2020.02.009I2

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