Streamline Cell Dissociation with High-Quality Reagents

Optimize your cell culture workflow with Atlantis Biosciences’ premium dissociation reagents. Partnering with Biowest, we offer a comprehensive selection of products for efficient and gentle disaggregation of tissues and monolayer cells.

Key Benefits:

Reliable results: Achieve consistent cell detachment with our high-quality reagents.
Versatile selection: Find the perfect solution for your specific cell types and applications.
Quality assured: Manufactured under strict ISO 9001:2008 and ISO 13485:2012 standards.
Sterile-filtered: Ensures aseptic cell culture conditions; 24 months shelf life.

Featured Dissociation Reagent: Trypsin EDTA

Trypsin EDTA is a widely used enzymatic solution derived from porcine pancreas. It effectively disaggregates anchorage-dependent mammalian cells and tissues. The optimal Trypsin concentration depends on cell sensitivity.

	Fast ;Effective	Gentle & Effective	Specialized for pharma/vaccine
Cat no	X0930-100	L0950-100	L0941-100
Animal origin	YES	NO	NO
High Cell Viablity	YES	YES	YES
Inactivation method by inhibitors	YES	NO	YES
Package size	100ml	100ml	100ml

Unsure which dissociation agent is right for you?

Contact our team of experts at [email protected] to discuss your specific needs.

Other packaging available on request

For your special formulation, please contact us, we can make your custom-made media on request

We deliver Biowest solutions across South East Asia (Singapore, Thailand, Vietnam, Indonesia, Philippines, Myanmar) and Across China Only.

Reference Publications

Beltrán-Camacho, L., Eslava-Alcón, S., Rojas-Torres, M. et al.

The serum of COVID-19 asymptomatic patients up-regulates proteins related to endothelial dysfunction and viral response in circulating angiogenic cells ex-vivo.

Mol Med 28, 40 (2022).

DOI: https://doi.org/10.1186/s10020-022-00465-w

Article Snippet: “After that, cells were collected using Trypsin–EDTA 1X (X0930-100; Biowest), centrifuged and washed once with PBS 1X, and snap frozen in liquid nitrogen before their storage at -80 ºC.”

Szymon Rutecki, Agnieszka Leśniewska-Bocianowska, Klaudia Chmielewska, Julia Matuszewska, Eryk Naumowicz, Paweł Uruski, Artur Radziemski, Justyna Mikuła-Pietrasik, Andrzej Tykarski, Krzysztof Książek

Serum starvation-based method of ovarian cancer cell dormancy induction and termination in vitro

Biology Methods and Protocols, Volume 8, Issue 1, 2023, bpad029

DOI: https://doi.org/10.1093/biomethods/bpad029

Article Snippet: “The tumors were cut into small fragments of comparable weight and then placed in 0.05% trypsin–0.02% EDTA (Biowest, cat. no. X0930) for 20 min at 37°C with shaking.”

Nielsen, S. S., Vibholm, L. K., Monrad, I., Olesen, R., Frattari, G. S., Pahus, M. H., Højen, J. F., Gunst, J. D., Erikstrup, C., Holleufer, A., Hartmann, R., Østergaard, L., Søgaard, O. S., Schleimann, M. H., & Tolstrup, M.

SARS-CoV-2 elicits robust adaptive immune responses regardless of disease severity.

EBioMedicine, 68, 103410. (2021a).

DOI: https://doi.org/10.1016/j.ebiom.2021.103410

Article Snippet: “Second, cell suspensions were made by incubating each well with 75μL Trypsin with EDTA(Biowest product code: X0930-100) for 15 min at 37°C, followed by centrifugation at 500 g for 5 min at room temperature, and re-suspension in DMEM containing 10% FBS and 50 U/mL P/S.”

Su, T., Chua, W., Liu, Y., Fan, J., Tan, S., Yang, D., & Sham, L. (2023).

Rewiring the pneumococcal capsule pathway for investigating glycosyltransferase specificity and genetic glycoengineering.

Science Advances, 9(36).

DOI: https://doi.org/10.1126/sciadv.adi8157

Article Snippet: “Cells were detached from the bottom of the culture bottle by washing them with 10 ml of 1× PBS (Vivantis, PB0344-1L) and incubated with 3 ml of 1× trypsin-EDTA solution diluted in 1× PBS (Biowest, X0930-100) for 5 min at 37°C in 5% CO₂.”

Camilla Siciliano, Antonella Bordin, Mohsen Ibrahim, Isotta Chimenti, Francesco Cassiano, Ilenia Gatto, Giorgio Mangino, Andrea Coccia, Selenia Miglietta, Daniela Bastianelli, Vincenzo Petrozza, Antonella Calogero, Giacomo Frati, Elena De Falco
The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots
Stem Cell Research, Volume 17, Issue 2, 2016, Pages 342-351, ISSN 1873-5061
DOI: https://doi.org/10.1016/j.scr.2016.07.010

Article Snippet: “Briefly, tissue specimens were enzymatically digested using 0.05% Trypsin/0.02% EDTA (Biowest, Nuaillé, France, Cat. N. X0930-100)/1 mg/ml collagenase type-I solution…”

Carducci A, Scafetta G, Siciliano C, Carnevale R, Rosa P, Coccia A, Mangino G, Bordin A, Vingolo EM, Pierelli L, Lendaro E, Ragona G, Frati G, De Falco E.

GMP-grade platelet lysate enhances proliferation and migration of tenon fibroblasts.

Front Biosci (Elite Ed). 2016 Jan 1;8(1):84-99.

DOI: https://doi.org/10.2741/E753

Article Snippet: “After reaching semi‑confluence, fibroblasts were trypsinized (0.0.5%Trypsin/0.0.2%EDTA, Cat. N. X0930‑100) and expanded with complete medium.”

Furdak P, Pieńkowska N, Bartosz G, Sadowska-Bartosz I.

Extracts of Common Vegetables Inhibit the Growth of Ovary Cancer Cells.

Foods. 2022; 11(16):2518.

DOI: https://doi.org/10.3390/foods11162518

Article Snippet: “The fetal bovine serum (FBS; cat. no. S1813), penicillin–streptomycin solution (cat. no. L0022), trypsin-EDTA solution (10x) (cat. no. X0930) and phosphate-buffered saline without Ca²⁺ and Mg²⁺ (cat. no. P0750) were obtained from Biowest (Nuaillé, France).”

Pieńkowska N, Bartosz G, Furdak P, Sadowska-Bartosz I.

Delphinidin Increases the Sensitivity of Ovarian Cancer Cell Lines to 3-bromopyruvate.

International Journal of Molecular Sciences. 2021; 22(2):709

DOI: https://doi.org/10.3390/ijms22020709

Article Snippet: “Fetal bovine serum (FBS; cat. no. S1813), penicillin–streptomycin solution (cat. no. L0022), trypsin-EDTA solution (10x) (cat. no. X0930) and phosphate-buffered saline without Ca²⁺ and Mg²⁺ (cat. no. P0750) were obtained from Biowest (Nuaillé, France).”

Grzegorz Bartosz, Natalia Pieńkowska, Izabela Sadowska-Bartosz,
Dosing metric in cellular experiments: The mol/cell metric has its limitations
Toxicology in Vitro, Volume 78, 2022, 105272, ISSN 0887-2333

DOI: https://doi.org/10.1016/j.tiv.2021.105272.

Article Snippet: “Cell culture media: Roswell Park Memorial Institute (RPMI) medium + GlutaMAX (cat. no 72400–021) and Dulbecco’s Modified Eagle Medium) (DMEM) + GlutaMAX (cat. no. 21885–025)) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fetal bovine serum (FBS; cat. no. S1813), penicillin–streptomycin solution (cat. no. L0022), trypsin-EDTA solution (10×) (cat. no. X0930)…”

Bartosz G, Pieńkowska N, Kut K, Cieniek B, Stefaniuk I, Sadowska-Bartosz I.

Effect of Low Concentration of Nitroxides on SH-SY5Y Cells Transfected with the Tau Protein.

International Journal of Molecular Sciences. 2023; 24(23):16675.

DOI: https://doi.org/10.3390/ijms242316675

Article Snippet: “Fetal bovine serum (cat. no. S1813), penicillin–streptomycin solution (cat. no. L0022), Trypsin–EDTA solution (10×) (cat. no. X0930), and phosphate-buffered saline without Ca2+ and Mg2+ (cat. no. P0750) were obtained from Biowest (Nuaillé, France).”