Selective KBM NK Cell Culture Kit – Kohjin Bio

Selective KBM NK Cell Culture Kit – Kohjin Bio

Selective KBM NK Cell Culture Kit – Kohjin Bio

$950.00

Unleash NK Cell Manufacturing Power! Selective KBM NK Cell Culture Kit efficiently expands NK cells from PBMC. Achieve high purity (close to 50%) & rapid growth (100-150x) in 10 days. Ideal for industrial applications!

Kohjin Bio KBM NK Kit for NK cell culture from PBMC

$950.00

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Boost Your NK Cell Manufacturing with Superior Efficiency and Purity:

Kohjin Bio’s selective KBM NK Cell Culture Kit from Kohjin Bio provides a streamlined solution for the industrial-scale production of highly pure NK cells for various applications. This optimized kit simplifies the expansion of functional NK cells from PBMCs, facilitating efficient and cost-effective cell therapy development.

Key Features:

  • Selective NK Cell Expansion: Effectively expands NK cells from PBMCs with high purity (almost 50%) in just 10 days, surpassing traditional methods.
  • Rapid Cell Growth: Achieve a 100-150 fold cell multiplication within 10 days, reaching a maximum of 3.6 billion cells.
  • Streamlined Workflow: The pre-coated culture bag and optimized media components minimize preparation steps and ensure consistent results.
  • Scalable Production: Designed for industrial use, this kit allows for easy scale-up to meet your client’s demands.

Benefits for Industrial Users:

  • Reduced Production Costs: Experience efficient NK cell expansion with minimal time and resource investment.
  • Enhanced Cell Quality: Obtain highly pure NK cell populations critical for effective progression plans towards clinical applications.
  • Reliable and Scalable Manufacturing: Ensure consistent and reproducible NK cell production for efficient cell therapy development.

 

Technical Specifications:

Components: KBM NKCC-b (culture bag), KBM NKCC-c (coating agent), KBM NKCC-1 (primary culture medium), KBM NKCC-2 (expansion culture medium)

Preservation: 2-8℃

 

Unleash the Potential of NK Cell Therapies:

The Selective KBM NK Cell Culture Kit empowers industrial researchers and manufacturers to unlock the potential of NK cell-based therapies. Contact us today at [email protected] to discuss your NK cell biomanufacturing needs.

Documents

Safety Data Sheet

KBM NK Kit Safety Data Sheet

 

KBM NK Kit Basic Protocol/Manual

KBM Kit Basic Manual

Sample Culture Protocol

Example of Culture Protocol

1. PBMC 2.0E+07 cells were seeded into T75 flasks pre-coated (NKCC-1 20mL, serum 2mL added) and incubated in a 5% CO2 incubator at 37℃.

2. After 5 days of culture, the cells were collected when the number of cells reachs to 8.2E+07 cells. Transfer to expansion culture (1200 rpm x 5 min).

3. Add 200 mL of NKCC-2 so that the cell concentration gets 4.0E+05 cells/mL. Add inactivated (56℃, 30 min) human serum (10 mL) obtained at the time of PBMC separation.

4. After 7 days of culture, add the remaining 800nL of NKCC-2 to the bag, after it was confirmed that the medium had become yellowish.

5. After 10 days of culture, count the number of cells, and confirm the cell surface markers (CD56, CD3) and measure the cytotoxic activity using K 562 (target cancer cell) on a flow cytometer.