PerCP-Cyanine5.5 Anti-Mouse CD4 (RM4-5)

PerCP-Cyanine5.5 Anti-Mouse CD4 (RM4-5)

PerCP-Cyanine5.5 Anti-Mouse CD4 (RM4-5)

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The RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will “compete” for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.

Recent Publications:
Bally APR, Lu P, Tang Y, Austin JW, Scharer CD, Ahmed R and Boss JM. 2015. J Immunol. doi: 10.4049/jimmunol.1402550. (Flow Cytometry)

Sekiya T, Kondo T, Shichita T, Morita R, Ichinose H and Yoshimura A. 2015. J Exp Med. 212(10): 1623-1640. (Flow Cytometry)

Product Details

Name PerCP-Cyanine5.5 Anti-Mouse CD4 (RM4-5)
Cat. No. 65-0042
Alternative Names L3T4, T4
Gene ID 12504
Clone RM4-5
Isotype Rat IgG2a, κ
Reactivity Mouse
Cross Reactivity
Format PerCP-Cyanine5.5
Application Flow Cytometry
Citations* Dolina JS, Lee J, Griswold RQ, Labarta-Bajo L, Kannan S, Greenbaum JA, Bahia El Idrissi N, Pont MJ, Croft M, Schoenberger SP. TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4+ T Cells to Treg. Cell Rep. 2020 Apr 7;31(1):107249. doi: 10.1016/j.celrep.2020.01.040.

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Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109:8670-8675. (Flow cytometry)

Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Immunofluorescence microscopy)

Poitrasson-Riviere M, Bienvenu B, Le Campion A, Becourt C, Martin B, and Lucas B. 2008. J. Immunol. 180:7294-7304. (Immunohistochemistry – paraffin embedded tissue)

Sorg H, Lorch B, Jaster R, Fitzner B, Ibrahim S, Holzhueter S, Nizze H, and Vollmar B. 2008. Am. J. Physio. Gastrointest. Liver Physiol. 295: G1274-1280. (Immunohistochemistry – paraffin embedded tissue)

Menke J, Lucas JA, Zeller GC, Keir ME, Huang XR, Tsuboi N, Mayadas TN, Lan HY, Sharpe AH, and Kelley VR. 2007. J. Immunol. 179: 7466-7477. (Immunohistochemistry – frozen tissue)

Irie J, Wu Y, Wicker LS, Rainbow D, Nalesnik MA, Hirsch R, Peterson LB, Leung PS, Cheng C, Mackay IR, Gershwin ME, and Ridgway WM. 2006. J Exp Med. 203(5):1209-19. (Immunohistochemistry – frozen tissue)

Bosselut R, Zhang W, Ashe JM, Kopacz JL, Samelson LE, and Singer A. 1999. J. Exp. Med. 190: 1517-1526. (Immunoprecipitation)

Shi Y, Kaliyaperumal A, Lu L, Southwood S, Sette A, Michaels MA, and Datta SK. 1998. J. Exp. Med. 187:367-378. (Blocking)

Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, and Hill TJ. 1995. J. Histochem. Cytochem. 43:313-320. (Immunohistochemistry – frozen tissue, zinc-fixed paraffin embedded tissue)

Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.

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