PE-Cyanine7 Anti-Human CD25 (BC96)

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The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells.

The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on “induced” peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.

Recent Publications:
Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T et al. 2015. Nat Commun. doi: 10.1038/ncomms9608. (Flow Cytometry)

Product Details

Name	PE-Cyanine7 Anti-Human CD25 (BC96)
Cat. No.	60-0259
Alternative Names	Interleukin-2 Receptor alpha, IL-2Rα, IL2Ra, Tac
Gene ID	3559
Clone	BC96
Isotype	Mouse IgG1, kappa
Reactivity	Human
Cross Reactivity	Baboon, Chimpanzee, Cynomolgus, Rhesus
Format	PE-Cyanine7
Application	Flow Cytometry
Citations*	Willer DO, Ambagala APN, Pilon R, chan JK, Fournier J, Brooks J, Sandstrom P, and MacDonald KS. 2012. J. Virol. 86: 3626-3634. (Flow cytometry – cynomolgus) Ruffell B, Au A, Rugo HS, Esserman LJ, Hwang ES, and Coussens LM. 2012. Proc. Natl. Acad. Sci. 109: 2796-2801. (Flow cytometry) Mackroth MS, Malhotra I, Mungai P, Koech D, Muchiri E, and King CL. 2011. J. Immunol. 186: 2780-2791. (Flow cytometry) Mori T, Miyamoto T, Yoshida H, Asakawa M, Kawasumi M, Kobayashi T, Morioka H, Chiba K, Toyama Y, and Yoshimura A. 2011. Int. Immunol. 23(11):701-712. (Cell sorting / negative selection – T cells) Wan Q, Kozhaya L, ElHed A, Ramesh R, Carlson TJ, Djretic IM, Sundrud MS, and Unutmax D. 2011. J. Exp. Med. 208: 1875-1887. (Cell sorting / negative selection – T cells) Verhoeven D, Sankaran S, Silvey M, and Dandekar S. 2008. J. Virol. 82: 4016-4027. (Flow cytometry – rhesus macaque)

Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.