PE Anti-Mouse CD86 (B7-2) (GL-1)

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The GL-1 antibody reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between ≥ ≥ ≥ the B7 ligands and CD28 provides crucial communication between ≥ ≥ ≥ T cells and B cells or APCs to coordinate the adaptive immune response.

The GL-1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.

Recent Publications:
Chaganty BKR, Lu Y, Qiu S, Somanchi SS, Lee DA and Fan Z. 2015. OncoImmunology. DOI: 10.1080/2162402X.2015.1100790. (Flow Cytometry)

Product Details

Name	PE Anti-Mouse CD86 (B7-2) (GL-1)
Cat. No.	50-0862
Alternative Names	B7.2, B70, Ly-58
Gene ID	12524
Clone	GL-1 (GL1)
Isotype	Rat IgG2a, κ
Reactivity	Mouse
Cross Reactivity
Format	PE
Application	Flow Cytometry
Citations*	Liu Z, Geboes K, Hellings P, Maerten P, Heremans H, Vandenberghe P, Boon L, van Kooten P, Rutgeerts P, and Ceuppens JL. 2011. J. Immunol. 167: 1830-1838. (in vivo blocking, Immunohistochemistry – OCT embedded frozen tissue) Kastenmuller W, Gasteiger G, Subramanian N, Sparwasser T, Busch DH, Belkaid Y, Drexler I, and Germain RN, 2011. J. Immunol. 187: 3186-3197. (in vivo blocking) Zheng SG, Wang JH, Stohl, W, Kim KS, Gray JD, and Horwitz DA. 2006. J. Immunol. 176:3321-3329. (in vitro blocking) Leithauser F, Meinhardt-Krajina T, Fink K, Wotschke B, Moller P and Reimann J. 2006. Am. J. Pathol. 168(6): 1898-1909. (Immunohistochemistry – frozen tissue) Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy – frozen tissue, Immunohistochemistry – frozen tissue) Lenschow DJ, Ho SC, Sattar H, Rhee L, Gray G, Nabavi N,Herold KC, and Bluestone JA. 1995. J. Exp. Med. 181:1145-155. (in vitro blocking) Blazar BR, Taylor PA, Panoskaltsis-Mortari A, Gray GS, and Vallera DA. 1995. Blood. 85: 2607-2618. (Immunohistochemistry – OCT embedded frozen tissue)

Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.