Iscove’s Modified Dulbecco’s Medium (IMDM) is a versatile cell culture medium specifically designed to support a wide range of cell types. As a modification of DMEM, IMDM contains additional nutrients, including selenium, amino acids, vitamins, sodium pyruvate, and HEPES buffer.

Key Features:

• Enhanced nutrient composition: Provides essential nutrients for optimal cell growth and function.
• Versatility: Supports a variety of cell types, including murine B lymphocytes, hemopoietic tissue, and hybrid cells.
• Buffered pH: HEPES buffer maintains stable pH for optimal cell culture conditions.
• Phenol red-free: Minimizes interference with light-sensitive assays.
• Stable glutamine: Ensures long-term stability and eliminates the need for supplemental glutamine.

Recommended Applications:

• Primary cell culture: Ideal for cultivating bone marrow-derived cells, B cells, and T lymphocytes.
• Hybridoma culture: Supports the growth and production of monoclonal antibodies.
• Cell line maintenance: Provides a suitable environment for various cell lines.

Storage and Handling:

• Store at 2-8°C, protected from light and humidity.
• Avoid repeated freeze-thaw cycles.
• Handle under aseptic conditions to prevent contamination.
• Supplement with sodium bicarbonate as needed.

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