1. Product Features
- · Mycoplasma contamination screening in investigational cell strains
- · Contains the main contaminants of the cell culture including M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, Acholeplasma laidlawii, allowing all mycoplasma detection of M. pneumoniae, M. salivarium, M. genitalium, M. penentrans and ureaplasma
- · The primer binds to the highly conserved 16s rRNA coding region
- · DNA of eukaryotes and other bacterial species are not detected
- · PCR performance monitoring is possible with the application of internal amplification control (IAC)
- · Optimized PCR Premix provided for easy use
- · Using the TaqMan probe method, real-time PCR (qPCR) technique is applied
(FAM labeled probe: Mycoplasma, HEX labeled probe: IAC) - · Main strand DNA preparation through boiling of the supernatant of the cell culture
- · Sensitivity: 1–100 fg/reaction
- · Result confirmation within 1–2 hr
2. Product results
3. Product type
Type | Product name | Cat. No. | Size |
---|---|---|---|
Mycoplasma screening (R&D) |
BioMycoX® Mycoplasma qPCR Detection Kit | QDR-25 | 25 Tests |
QDR-50 | 50 Tests | ||
QDR-100 | 100 Tests |
4. User manual