1. Product Features
- · Mycoplasma contamination screening in R&D cell lines
- · Contains the main contaminants of the cell culture including M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, Acholeplasma laidlawii, allowing all mycoplasma detection of M. pneumoniae, M. salivarium, M. genitalium, M. penentrans and ureaplasma
- · The primer binds to the highly conserved 16s rRNA coding region
- · DNA of eukaryotes and other bacterial species are not detected
- · PCR performance monitoring is possible with the application of internal amplification control (IAC)
- · Optimized PCR Premix provided for easy-to-use
- · Conventional PCR technique applied
- · Template DNA preparation through boiling of the supernatant of the cell culture
- · Sensitivity: 10–100 fg/reaction
- · The test results are within 2~3 hours
An approximate of 250–300 bp DNA band can be observed at mycoplasma contamination. Furthermore, when PCR reaction is performed appropriately, an internal DNA band of an approximately 700 bp can be identified.
3. Product type
Type | Product name | Cat. No. | Size |
---|---|---|---|
Mycoplasma screening (R&D) |
BioMycoX® Mycoplasma PCR Detection Kit | D-25 | 25 Tests |
D-50 | 50 Tests | ||
D-100 | 100 Tests | ||
SafeDry™ Mycoplasma PCR Detection Kit* | LD-8 | 8 Tests (8 strip x 1 ea) |
|
LD-48 | 48 Tests (8 strip x 6 ea) |
* SafeDryTM PCR Detection Kit is freeze-dried form of BioMycoX® Mycoplasma PCR Detection Kit.
4. User manual