NucSpot® Live Cell Nuclear Stains

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Biotium products are distributed only in Singapore and Thailand.

NucSpot® Live Cell Nuclear Stains are cell membrane-permeant DNA dyes that specifically stain nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, with low toxicity for live cell imaging.

Features

No-wash, nuclear-specific DNA dyes
Low-toxicity for real-time live cell imaging
Fix before or after labeling
NucSpot® Live 650 compatible with SIM, STED, or STORM
Choose green or far-red fluorescence

Kit Components

NucSpot® Live Dye, 1000X in DMSO
Verapamil, 100 mM in DMSO

Spectral Properties

NucSpot® Live 488 Ex/Em: 500/515 nm
NucSpot® Live 650 Ex/Em: 650/675 nm

NucSpot® Live 488 stains nuclei with green fluorescence for the FITC channel, while NucSpot® Live 650 has far-red fluorescence for the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has been validated in STORM (Ref. 4). The dyes are supplied as 1000X stock solutions in DMSO, and are supplied with a vial of the efflux pump inhibitor verapamil for optional use, which may increase probe retention and live cell staining in some cell types. Note: NucSpot® Live 488 and NucSpot® Live 650 have dim blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.

NucSpot® Live Cell Nuclear Stains can be used to stain live gram-positive bacteria, but do not stain live gram-negative bacteria or yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Also see our wide selection of other Membrane and Organelle Stains, or download the Cellular Stains Brochure.

Cy Dye is a registered trademark of GE Healthcare.

PRODUCT ATTRIBUTES

Dye	NucSpot® Live 488, NucSpot® Live 650
Cellular localization	Nucleus
For live or fixed cells	For fixed cells, For live/intact cells
Assay type/options	Long term staining (24-72h), No-wash staining, Real-time imaging
Cell permeability	Membrane permeant
Colors	Green, Far-red
Fixation options	Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

REFERENCES

BBRC (2018) 507, 496e502. https://doi.org/10.1016/j.bbrc.2018.11.071 (40081)
Translational Oncol (2018), 11(3), 700-714. https://doi.org/10.1016/j.tranon.2018.03.003 (40081, EVOS FL Imager)
bioRxiv (2019), https://doi.org/10.1101/672147 (40081)
bioRxiv (2019), https://www.biorxiv.org/content/10.1101/559484v2 (40082, STORM)
Clin Cancer Res (2019) 25(14), 4332-4342. doi:10.1158/1078-0432.CCR-18-3312 (40081, in mounting medium)
Int J Mol Sci (2018) 19, 1870. doi:10.3390/ijms19071870 (40081)
Reprod Sci (2020) 27(3), 779-786. doi: 10.1007/s43032-019-00102-8 (40081)
FEBS Open Bio (2020). https://doi.org/10.1002/2211-5463.13059 (40082)
J Cell Phys (2021), jcp.30336. doi: 10.1002/jcp.30336 (40081)
Pharmaceutics 2021, 13, 10. https://dx.doi.org/10.3390/pharmaceutics13010010 (40082)
J Cell Physiol (2021), 1–12. DOI: 10.1002/jcp.30336 (40081, 3D cell culture)

NucSpot® Live Cell Nuclear Stains