5-CFDA (5-Carboxyfluorescein diacetate, single isomer)
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5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).
- Off-white solid soluble in DMSO
- Store desiccated at 4°C
- C25H16O9
- MW: 460
- [79955-27-4]
PRODUCT ATTRIBUTES
| CAS number | 79955-27-4 |
|---|---|
| Applications | pH indicators |
| Cell permeability | Membrane permeant |
| Cellular localization | Nucleus & cytoplasm |
| pKa | pKa 6.5 |
| Indicator type | Ratiometric |
| Colors | Green |
| Excitation/Emission | 495/519 nm (pH 9) (after hydrolysis) |
REFERENCES
1. J Cell Sci 98, 343(1991).
2. Nature 295, 524 (1982).
3. J Immunol Meth 100, 261 (1987).
4. J Immunol Meth 33, 33 (1980).
PROTOCOLS
SDS
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