LysoView™ Dyes
Bright no-wash fluorescent dyes for live-cell lysosome imaging. Designed for high specificity, low background and long-term imaging, with colours from blue to near-IR and options validated for super-resolution microscopy. Ideal for studying lysosomal function, autophagy and cell health.
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LysoView™ Dyes – No-Wash Lysosomal Stains for Live-Cell Imaging
LysoView™ dyes are advanced fluorescent probes designed for high-specificity lysosome staining in live cells, offering bright, low-background fluorescence without a wash step. Available in a full spectrum from blue to near-infrared, LysoView™ provides flexible options for confocal, SIM, STED and long-term live-cell imaging.
Perfect for researchers studying lysosomal biology, autophagy, cell stress, neurodegeneration and drug response.
Key Benefits
- No-wash lysosome staining for clean, high-contrast images
- Non-toxic formulation supports real-time and long-term imaging (24–72 h)
- Brighter and lower background compared to conventional lysosomal probes
- Validated for super-resolution: LysoView™ 488 (SIM), LysoView™ 650 (SIM and STED)
- pH-sensitive options (LysoView™ 540, LysoView™ 633) for enhanced specificity
- Wide colour range: blue, green, orange, red, far-red and unique NIR
- Flexible trial sizes for convenient assay testing
- Cost-efficient per assay with concentrated 1000X format
Product Features
- Targets lysosomes in live/intact cells
- Membrane-permeant fluorescence for immediate staining
- Compatible with super-resolution microscopy
- Photostable dyes (550, 594, 640, 650) ideal for prolonged imaging
- Storage at −10 to −35 °C, light-protected
- Available in 10 µL trials, 50 µL vials and lyophilised formats
Applications
- Lysosome localisation and morphology studies
- Autophagy and endolysosomal dynamics
- Drug-induced lysosomal perturbation
- High-content screening
- Super-resolution lysosomal mapping
Specification
| Attribute | Specification |
|---|---|
| Size | Trial size (10 µL), 50 µL, Trial size (1 vial), Set of 10 vials |
| Probe cellular localisation | Lysosomes |
| Dye | LysoView™ 405, LysoView™ 488, LysoView™ 540, LysoView™ 550, LysoView™ 594, LysoView™ 633, LysoView™ 640, LysoView™ 650, LysoView™ 680 |
| For live or fixed cells | For live/intact cells |
| Assay type/options | Long-term staining (24–72 h), No-wash staining, Real-time imaging |
| Cell permeability | Membrane permeant |
| Colours | Blue, Green, Orange, Red, Far-red, Near-infrared |
| Storage conditions | Store at −10 to −35 °C, Protect from light |
Variations
| Product | Catalogue Number | Size | Abs/Em (nm) pH ≤ 5 | Detection Channel | Features |
|---|---|---|---|---|---|
| LysoView™ 405, 1000X in DMSO | 70066-T | 10 µL | 318, 400 / 464 | DAPI, Pacific Blue™ | Blue fluorescent lysosome stain |
| 70066 | 50 µL | ||||
| LysoView™ 488, 1000X in DMSO | 70067-T | 10 µL | 496 / 526 | GFP, FITC | Green fluorescent dye validated in SIM |
| 70067 | 50 µL | ||||
| LysoView™ 540, 1000X in DMSO | 70061-T | 10 µL | 540 / 634 | TRITC, Cy®3, PE | Orange, pH-sensitive fluorescence** |
| 70061 | 50 µL | ||||
| LysoView™ 550, 1000X in DMSO | 70083-T | 10 µL | 542 / 567 | TRITC, Cy®3, PE | Bright and photostable orange dye |
| 70083 | 50 µL | ||||
| LysoView™ 594, 1000X in DMSO | 70084-T | 10 µL | 585 / 634 | Texas Red® | Bright and photostable red dye |
| 70084 | 50 µL | ||||
| LysoView™ 633 (lyophilised solid) | 70058-T | 1 vial* | 634 / 657 | Cy®5, APC | Far-red, pH-sensitive fluorescence |
| 70058 | 10 vials* | ||||
| LysoView™ 640, 1000X in DMSO | 70085-T | 10 µL | 640 / 671 | Cy®5, APC | Bright and photostable far-red dye |
| 70085 | 50 µL | ||||
| LysoView™ 650, 1000X in DMSO | 70059-T | 10 µL | 650 / 675 | Cy®5, APC | Photostable far-red dye compatible with SIM, STED |
| 70059 | 50 µL | ||||
| LysoView™ 680, 1000X in DMSO | 70086-T | 10 µL | 674 / 711 | Cy®5.5 | Unique near-IR lysosome stain |
| 70086 | 50 µL |
Citations
1. Wang X, Xu P, … De Camilli P.
The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage.
Nature Cell Biology (2025).
Reagent use: LysoView™ 633 (Biotium, 70058; 1:2,000), LysoView™ 640 (Biotium, 70085; 1:2,000).
2. Beckwith KS, Beckwith MS, … Flo TH.
Plasma membrane damage causes NLRP3 activation and pyroptosis during Mycobacterium tuberculosis infection.
Nature Communications (2020).
Reagent use: LysoView™ 633 (Biotium, 70058).
3. Manna S, Howitz WJ, … Esser-Kahn AP.
Immunomodulation of the NLRP3 inflammasome through structure-based activator design and functional regulation via lysosomal pathways.
ACS Central Science (2018).
Reagent use: LysoView™ 633 (Biotium, 70058).
4. Wang H, Tam YYC, … Cullis PR.
The Niemann–Pick C1 inhibitor NP3.47 enhances gene silencing potency of lipid nanoparticles containing siRNA.
Molecular Therapy (2016).
Reagent use: LysoView™ 633 (Biotium; 70058).
5. Augenstreich J, Phan AT, … Briken V.
Dynamic interplay of autophagy and membrane repair during Mycobacterium tuberculosis infection.
PLoS Pathogens (2025).
Reagent note: LysoView™ probe referenced (Biotium; Cat#70058), with discussion of pH-dependent fluorescence.
6. Wang C-I, Chen C-Y, … Chang K-P.
PSMA2 promotes chemo- and radioresistance of oral squamous cell carcinoma by modulating mitophagy pathway.
Cell Death Discovery (2025).
Reagent use: LysoView™ (green) for lysosomal imaging; co-localisation studies with MitoView™.
