High-Quality Classical Media for Optimal Cell Culture

Partnering with Biowest, we offer a comprehensive selection of trusted media formulations, including Dulbecco’s Modified Eagle Medium (DMEM), Eagle’s Minimum Essential Medium (EMEM), RPMI 1640, and Ham’s F-12. Our products are meticulously manufactured in state-of-the-art ISO certified facilities to ensure consistent quality and reliability.

Key Features:

RPMI 1640 w/ L-Glutamine: Our most popular RPMI formulation
Versatile formulations: Choose from a variety of classical media to suit your specific cell culture needs.
Proven performance: Compatible with a wide range of cell lines, including those from ATCC, DSMZ, and Riken.
Exceptional quality: Manufactured in ISO 9001:2008 and ISO 13485:2012 certified facilities.
Extended shelf life: Sterile-filtered media with a 12-month shelf life for convenience.
Trusted by leading institutions: Widely used in renowned research centers like NUS and CSI in Singapore.

** please inquiry for powdered formulation

Please enquire with us at [email protected]

We deliver Biowest solutions across South East Asia (Singapore, Thailand, Vietnam, Indonesia, Philippines, Myanmar) and Across China Only.

Reference Publications

Mostoufi-Afshar, S., Tabatabaei, M., & Ghahramani Seno, M. M.

Mycobacterium avium subsp. paratuberculosis induces differential cytosine methylation at miR-21 transcription start site region

Iranian journal of veterinary research, 19(4), 262–269

DOI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361596/

Article Snippet: “The MAP cells were washed twice in plain RPMI1640 (Cat. No. L0500-500, Biowest, France) and resuspended in 1 ml of plain RPMI1640 by vortexing”

Cosín-Tomàs M, Senserrich J, Arumí-Planas M, Alquézar C, Pallàs M, Martín-Requero Á, Suñol C, Kaliman P, Sanfeliu C.

Role of Resveratrol and Selenium on Oxidative Stress and Expression of Antioxidant and Anti-Aging Genes in Immortalized Lymphocytes from Alzheimer’s Disease Patients

Nutrients. 2019; 11(8):1764.

DOI: https://doi.org/10.3390/nu11081764

Article Snippet: “The cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 (Biowest, Nuaillé, France, #L0500), which contained 2 mM L-glutamine, supplemented with 10% fetal bovine serum”

Ciaffaglione V, Consoli V, Intagliata S, Marrazzo A, Romeo G, Pittalà V, Greish K, Vanella L, Floresta G, Rescifina A, et al.

Novel Tyrosine Kinase Inhibitors to Target Chronic Myeloid Leukemia

Molecules. 2022; 27(10):3220.

DOI: https://doi.org/10.3390/molecules27103220

Article Snippet: “K562 cells (CCL-243 ATCC) were cultured in RPMI 1640 (L0500, Biowest, Riverside, MO, USA) supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin–streptomycin and incubated at 37 °C in 5% CO₂.”

Mohammadi Abandansari R, Parsian H, Kazerouni F, Porbagher R, Zabihi E, et al.

Effect of Simultaneous Treatment with Royal Jelly and Doxorubicin on the Survival of the Prostate Cancer Cell Line (PC3): An In Vitro Study

Int J Cancer Manag. 2018;11(4):e13780.

DOI: https://doi.org/10.5812/ijcm.13780.

Article Snippet: “PC3 cells were provided by the Pasture Institute (Tehran, Iran) and cultured in Roswell Park Memorial Institute (RPMI) 1640 culture medium (Biowest L0500 – 1000) containing 15% fetal bovine serum (FBS) (Biowest L1820 – 100) and penicillin-streptomycin 1% (Sigma P4333 – 100 mL).”

Niederauer, C., Nguyen, C., Wang-Henderson, M. et al.

Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions

Nat Commun 14, 4345 (2023).

DOI: https://doi.org/10.1038/s41467-023-40065-8

Article Snippet: “Cell biology media and supplements used were DMEM without Phenol Red (12-917F), DMEM with Phenol Red (12-604F, Lonza), RPMI 1640 with Phenol Red (L0500-500, biowest), RPMI 1640 without Phenol Red (11835-030, gibco).”

Enrica E.K. Tan, Richard A. Hopkins, Chrissie K. Lim, Saumya S. Jamuar, Christina Ong, Koh C. Thoon, et al.

Dominant-negative NFKBIA mutation promotes IL-1β production causing hepatic disease with severe immunodeficiency

J Clin Invest. 2020;130(11):5817-5832.

DOI: https://doi.org/10.1172/JCI98882.

Article Snippet: “Cells were differentiated in culture for 7 days with 20 ng/mL M-CSF (416-ML-50, R&D Systems) in RPMI 1640 media (L0500, Biowest) supplemented with 10% FBS …”

Teixido, Cristina & Marés, Rosó & Aracil, Miguel & Ramon Y Cajal, Santiago & Hernández-Losa, Javier.

Epithelial-Mesenchymal Transition Markers and HER3 Expression Are Predictors of Elisidepsin Treatment Response in Breast and Pancreatic Cancer Cell Lines

PloS one. 8. e53645.

DOI: 10.3390/ijms24032838

Article Snippet: “Both cell lines overexpress EpCAM [62,63] and the BxPC3 cell line overexpresses HER3 [64,65]. BxPC3 cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (L0500, Biowest) supplemented with 2 mM L-glutamine, 10% fetal bovine serum (FBS; F7524, Sigma-Aldrich), and 1% penicillin-streptomycin (L0022, Biowest, Berlin, Germany).”