DiO lipophilic tracer lipophilic carbocyanine dye

Biotium products are distributed only in Singapore and Thailand.

Visualize Cell Dynamics with DiO: The Classic Lipophilic Tracer

Biotium’s DiO stain offers a reliable and versatile option for labeling cell membranes in your research. This green fluorescent, lipophilic carbocyanine dye is a well-established tool for tracing cellular movement and interactions.

Key Features of DiO:

Widely Used Tracer: DiO is a popular choice for lipophilic tracing experiments due to its well-documented performance and ease of use.
Bright Green Fluorescence: The λEx/λEm maxima at 484/501 nm provides clear and distinct labeling for accurate image analysis.
Compatible with DiI: Combine DiO with DiI for dual-color labeling and colocalization studies, expanding your experimental capabilities.
Multiple Formats: Choose from the versatile DiO powder or the ready-to-use CellBrite™ Green solution for simplified workflow integration.

Please note:

DiO exhibits lower solubility and a slower lateral diffusion rate compared to newer alternatives like Neuro-DiO.
For applications requiring superior solubility and faster diffusion, consider exploring Neuro-DiO (Product #60015).

DiO Applications:

Live cell imaging of cell migration and morphology
Investigating cell-cell interactions
Monitoring neurite outgrowth
Multicolor colocalization studies (in combination with DiI)

Technical Specifications

CAS number: 34215-57-1

Probe cellular localization: Membrane/cell surface, Membrane/vesicular

For live or fixed cells: For fixed cells, For live/intact cells

Assay type/options: Co-cultures, Extended staining (several days to weeks)

Fixation options: Permeabilize before staining, Fix after staining (formaldehyde), Fix before staining (formaldehyde)

Colors: Green

Excitation/Emission: 484/501 nm

λEx/λEm (MeOH): 484/501 nm

Extinction Coefficient (ε): 150,000

Appearance: Yellow orange solid

Solubility: DMF, DMSO, 1:1 DMSO:ethanol (limited)

Storage: 4°C, protected from light

Reference Publications

Alu Konno, Naoya Matsumoto, Yasuko Tomono, and Shigetoshi Okazaki

Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures

Scientific reports vol. 10,1 12613. 28 Jul. 2020

DOI: 10.1038/s41598-020-69394-0

Article Snippet: “We tested several commercially available DiI analogues and successfully introduced three additional dyes with green (DiO), deep red (DiD), and near-infrared (DiR) fluorescence to this technique to increase the number of choices for available colours.”

Elie Beit‐Yannai, Saray Tabak, and W. Daniel Stamer

Physical exosome:exosome interactions

Journal of cellular and molecular medicine vol. 22,3 (2018): 2001-2006.

DOI: 10.1111/jcmm.13479

Article Snippet: “while extracellular vesicles from NTM cells were suspended in the same amount of PBS containing 7.5 μl DiO (3,3′ ‐dioctadecyloxacarbocyanine, perchlorate).”

Janna L. Crossley, et al.

Itaconate-producing neutrophils regulate local and systemic inflammation following trauma

JCI Insight. 2023;8(20):e169208

DOI: https://doi.org/10.1172/jci.insight.169208.

Article Snippet: “Following histopaque-mediated neutrophil isolation from the HO site 7 days after burn/tenotomy in WT mice, cell membranes were labeled with the DiO Lipophilic Carbocyanine fluorescent green dye kit (Biotium, 60011).”