KBM Neural Stem Cell 500ml

KBM Neural Stem Cell: Serum-Free Medium for Neural Stem Cells

KBM Neural Stem Cell is a specialized, serum-free, and chemically-defined medium meticulously developed for research and development pertaining to neural stem cells (NSCs) and neural cells. It is particularly optimized for the neurosphere assay, a widely utilized method in neuroscience research. This medium facilitates the robust culture of NSCs as neurospheres when combined with KBM Neural Stem Cell Supplement and employed in suspension culture. Furthermore, it supports the subsequent generation of new neurospheres through cell dispersion and re-culture.

Beyond proliferation, KBM Neural Stem Cell also possesses the capability to induce the differentiation of NSCs into various neural cell types through adherent cell cultures of neurospheres, without the requirement of additional supplement. This innovative medium was developed and commercialized in collaboration with Professor Hideyuki Okano, MD, PhD, from the Department of Physiology, Graduate School of Medicine, Keio University

Key Features:

Neurosphere culture: Supports the formation and growth of neurospheres.
Differentiation induction: Facilitates the differentiation of NSCs into neural cells.
Chemically-Defined & Serum-Free: The medium is entirely serum-free and chemically-defined, ensuring consistent and reproducible culture conditions.
Dual Functionality: It supports both the undifferentiated proliferation of NSCs (when used with KBM Neural Stem Cell Supplement) and the induction of their differentiation into neural cells (without the supplement).
Human iPS Cell Compatibility: KBM Neural Stem Cell has been validated for its capability to culture NSCs derived from human induced pluripotent stem (iPS) cells.
Comprehensive System: The product system includes KBM Neural Stem Cell medium (500 mL) and the concentrated (50x) KBM Neural Stem Cell Supplement (1 mL or 10 mL), which is also chemically-defined and contains retinoic acid (vitamin A).

Recommended Applications:

Neural stem cell research: Studying the biology and potential therapeutic applications of NSCs.
Neurosphere assays: Quantifying NSC proliferation and differentiation.
Drug discovery: Identifying compounds that modulate NSC function.

Graphs showing population doubling time and total cell number of neural stem cells across passages, and a microscope image of neurospheres. — Expansion of Neural Stem Cells in Suspension Culture with KBM Neural Stem Cell + Supplement

Immunofluorescence image of differentiated neurons stained green, blue, and red, cultured on a coated glass surface. — Differentiation of Neural Stem Cells in Adherent Culture Using KBM Neural Stem Cell Medium

Fluorescence image of neurons expressing TuJ-1 marker, cultured with KBM Neural Stem Cell medium and XB2. — Neural Differentiation Enhanced with KBM XB2 Supplement

Product Catalogue

Product No.	Product Name	Size	Shelf Life	Storage
16050100	KBM Neural Stem Cell	500 mL	1 year	-20℃
16050300	KBM Neural Stem Cell Supplement	1 mL	3 years	-20℃
16050400	KBM XB2	10 mL	2 years	-20℃

Publication

Shimojo, D., Onodera, K., Doi-Torii, Y. et al.

Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells.

Mol Brain 8, 79 (2015).

DOI: https://doi.org/10.1186/s13041-015-0172-4

Article Snippet: “…coated dishes at a density of 5×10⁴–1×10⁵ cells/cm² and cultured in motor neuron medium (MNM) consisting of media hormone mix (MHM) or KBM Neural Stem medium (Kohjin Bio, Japan) …”

Kitajima, R., Nakai, R., Imamura, T., Kameda, T., Kozuka, D., Hirai, H., Ito, H., Imai, H., & Imamura, M.

Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells.

Stem Cell Research, 44, 101749. (2020).

DOI: https://doi.org/10.1016/j.scr.2020.101749

Article Snippet: “Briefly, chimpanzee iPSCs were dissociated with TrypLE Express and 1.5 × 10⁵ cells/ml were transferred to 6-well low attachment culture plates in KBM Neural Stem Cell medium (16050200; Kohjin Bio, Saitama, Japan) supplemented with 10 μM Y-27632. “

Hara, T., Maejima, I., Akuzawa, T., Hirai, R., Kobayashi, H., Tsukamoto, S., Tsunoda, M., Ono, A., Yamakoshi, S., Oikawa, S., & Sato, K.

Rer1-mediated quality control system is required for neural stem cell maintenance during cerebral cortex development.

PLoS Genetics, 14(9), e1007647. (2018).

DOI: https://doi.org/10.1371/journal.pgen.1007647

Article Snippet: “The isolated cells were plated at a density of 2 × 105 cells/mL in KBM Neural Stem Cell (KOHJIN BIO #16050100) with KBM neural stem cell supplement containing epidermal growth factor and fibroblast growth factor (KOHJIN BIO) and cultured at 37°C in 5% CO2″

Odaka, H., Numakawa, T., Yoshimura, A., Nakajima, S., Adachi, N., Ooshima, Y., Inoue, T., & Kunugi, H.

Chronic glucocorticoid exposure suppressed the differentiation and survival of embryonic neural stem/progenitor cells: Possible involvement of ERK and PI3K/Akt signaling in the neuronal differentiation.

Neuroscience Research, 113, 28–36. (2016).

DOI: https://doi.org/10.1016/j.neures.2016.07.002

Article Snippet: “Then, the cells were diluted with proliferation medium (KBM neural stem cell medium from KOHJIN BIO, Saitama, Japan) containing KBM neural stem cell supplement…”

Kamata, Y., Isoda, M., Sanosaka, T., Shibata, R., Ito, S., Okubo, T., Shinozaki, M., Inoue, M., Koya, I., Shibata, S., Shindo, T., Matsumoto, M., Nakamura, M., Okano, H., Nagoshi, N., & Kohyama, J.

A robust culture system to generate neural progenitors with gliogenic competence from clinically relevant induced pluripotent stem cells for treatment of spinal cord injury.

Stem Cells Translational Medicine, 10(3), 398–413. (2020).

DOI: https://doi.org/10.1002/sctm.20-0269

Article Snippet: “Undissociated 15 to 20 neurospheres were plated on 8-well chamber glass slides coated with 1% growth factor reduced Matrigel (Corning), and cultured in differentiation medium that consisted of KBM Neural Stem Cell (KOHJIN BIO) supplemented with 2% B27, 1% nonessential amino acids (Thermo Fisher Scientific), 60 ng/mL T3, 10 ng/mL NT-3, 10 ng/mL Leukemia Inhibitory Factor (Millipore), and 25 ng/mL Ciliary Neurotrophic Factor (Peprotech) for 4 weeks in a humidified atmosphere of 5% CO₂. “

Onode, E., Uemura, T., Takamatsu, K. et al.

Bioabsorbable nerve conduits three-dimensionally coated with human induced pluripotent stem cell-derived neural stem/progenitor cells promote peripheral nerve regeneration in rats.

Sci Rep 11, 4204 (2021).

DOI: https://doi.org/10.1038/s41598-021-83385-9

Article Snippet: “Then, nerve conduits were placed and incubated in KBM Neural Stem Cell medium (Kohjin Bio, Saitama, Japan), supplemented with MACS NeuroBrew-21 (Miltenyi Biotec, Bergisch Gladbach, Germany), Leukemia Inhibitory Factor human (Merck, Darmstadt, Germany), and recombinant human FGF-basic (Pepro Tech, Rocky Hill, New Jersey, USA) for 14 days.”

Onodera, K., Shimojo, D., Ishihara, Y. et al.

Unveiling synapse pathology in spinal bulbar muscular atrophy by genome-wide transcriptome analysis of purified motor neurons derived from disease specific iPSCs.

Mol Brain 13, 18 (2020).

DOI: https://doi.org/10.1186/s13041-020-0561-1

Article Snippet: “…coated dishes at a density of 5 × 10⁴–1 × 10⁵ cells/cm² and cultured in motor neuron medium (MNM) consisting of media hormone mix (MHM) or KBM Neural Stem Cell medium (Kohjin Bio, Japan) [58] supplemented with 2% B27 supplement (Thermo Fisher Scientific, USA), 1% NEAA, 50 nM RA, 500 nM purmorphamine, 10 μM cyclic AMP…”

Chai, M., Sanosaka, T., Okuno, H., Zhou, Z., Koya, I., Banno, S., Andoh-Noda, T., Tabata, Y., Shimamura, R., Hayashi, T., Ebisawa, M., Sasagawa, Y., Nikaido, I., Okano, H., & Kohyama, J.

Chromatin remodeler CHD7 regulates the stem cell identity of human neural progenitors.

Genes & Development, 32(2), 165–180. (2018).

DOI: https://doi.org/10.1101/gad.301887.117

Article Snippet: “.…dishes in KBM neural stem cell medium (Kohjin Bio) containing B27 (1:50) supplemented with 1 µM CHIR99021. The next day, the medium was replaced with KBM neural stem cell medium supplemented with 10 ng/mL BDNF (R&D),…”

Nakai, K., Shiga, T., Yasuhara, R. et al.

In vitro monitoring of HTR2A-positive neurons derived from human-induced pluripotent stem cells.

Sci Rep 11, 15437 (2021).

DOI: https://doi.org/10.1038/s41598-021-95041-3

Article Snippet: “The day when the medium was changed to KBM medium, was defined as day in vitro (DIV) 0.”

Yoshimura, A., Adachi, N., Matsuno, H., Kawamata, M., Yoshioka, Y., Kikuchi, H., Odaka, H., Numakawa, T., Kunugi, H., Ochiya, T., & Tamai, Y.

The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles.

Disease Models & Mechanisms, 11(1). (2018).

DOI: https://doi.org/10.1242/dmm.028779

Article Snippet: “Isolated eNSCs were cultured on noncoated tissue culture plastic as neurospheres in KBM neural stem cell proliferation medium (Kohjin Bio, Saitama, Japan) with 0.2% KBM supplement containing EGF and bFGF (Kohjin Bio) at 37°C in air enriched with 5% CO2, as described previously (Yoshimura et al., 2016a).”