KBM591

KBM591

KBM591

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KBM591 is a versatile cell culture medium specifically designed for the activation and expansion of human lymphocytes.

This serum-free, xeno-free formulation provides an optimal environment for T cell and NK cell culture. 

Key Features:

  • Versatility: Supports both T cell and NK cell culture.
  • Xeno-free: Eliminates the need for animal-derived components.
  • Serum-free: Offers a defined and controlled culture environment.
  • Customizable: Can be supplemented with serum for specific applications.
  • Antibiotic-free: Provides flexibility for adding desired antibiotics.

 

Recommended Applications:

  • Lymphocyte research: Studying T cell and NK cell function and activation.
  • Immunotherapy: Developing cell-based therapies for various diseases.

 

Proliferation for T cell

【Cells】 Human peripheral blood

【Medium】KBM 591

【Vessel】 24 well plate coated with anti-CD3

【Protocol】Seeded 4×105 cells/well and incubated statically at 37℃・5% carbon dioxide.

Added KBM591(with IL-2) according to growth, and cultured for 14 days.

 

Fig. 3: NK cell Proliferative Response

 

Fig. 2: CD3 Positive Rate

 

Proliferation for NK cell

【Cells】 Human peripheral blood

【Medium】KBM 591

【Vessel】 24 well plate coated with NKCC-c

【Protocol】 Seeded 1×106 cells/well and incubated statically at 37℃・5% carbon dioxide.

Added KBM591(with IL-2) according to growth, and cultured for 14 days.

 

Fig. 3: NK cell Proliferative Response

 

Fig. 4: NK cell Marker Ratio

 

Documents

KBM591 English