KBM591 is a versatile cell culture medium specifically designed for the activation and expansion of human lymphocytes.
This serum-free, xeno-free formulation provides an optimal environment for T cell and NK cell culture.
Key Features:
- Versatility: Supports both T cell and NK cell culture.
- Xeno-free: Eliminates the need for animal-derived components.
- Serum-free: Offers a defined and controlled culture environment.
- Customizable: Can be supplemented with serum for specific applications.
- Antibiotic-free: Provides flexibility for adding desired antibiotics.
Recommended Applications:
- Lymphocyte research: Studying T cell and NK cell function and activation.
- Immunotherapy: Developing cell-based therapies for various diseases.
Proliferation for T cell
【Cells】 Human peripheral blood
【Medium】KBM 591
【Vessel】 24 well plate coated with anti-CD3
【Protocol】Seeded 4×105 cells/well and incubated statically at 37℃・5% carbon dioxide.
Added KBM591(with IL-2) according to growth, and cultured for 14 days.
Proliferation for NK cell
【Cells】 Human peripheral blood
【Medium】KBM 591
【Vessel】 24 well plate coated with NKCC-c
【Protocol】 Seeded 1×106 cells/well and incubated statically at 37℃・5% carbon dioxide.
Added KBM591(with IL-2) according to growth, and cultured for 14 days.