One-Step Blue® Protein Gel Stain – 1L

One-Step Blue® Protein Gel Stain – 1L

A visible, blue protein gel staining solution. Fast protein staining in a single step without fixation or washing.

Product Description

One-Step Blue® Protein Gel Stain is a ready-to-use visible blue protein gel staining solution. With no fixation or wash steps required, One-Step Blue® is a great choice for convenience and safety. One-Step Blue® can detect as little as approximately 10-20 ng of protein per band.

  • One-step staining procedure, no fixation required
  • Non-toxic formulation is safe for easy disposal
  • Sensitive protein detection down to 10-20 ng
  • Visualize blue bands, or image with a near-infrared fluorescence imager
  • 1 L size
  • Store at 4°C

Fast and simple procedure

One-Step Blue® produces fast (5-60 min) protein staining in a single step without fixation or washing. Destaining is optional. One-Step Blue® has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence.

One-Step Blue® is non-toxic

One-Step Blue® offers safer handling and disposal compared to Coomassie staining because it is entirely aqueous-based, without hazardous methanol or acetic acid.  One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

One-Step Blue™ is an alternative to tedious Coomassie staining, and can be imaged visually or using a near-infrared fluorescence imager.

Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ and One-Step Blue™, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue staining.

Product Replacement for Catalog number Unit size Product protocol Safety report
One-Step Blue® Coomassie stain 21003-1L 1L PI-21003 One-Step Gel Stains Safety Report
One-Step Lumitein™ SYPRO® Ruby Protein Gel Stain 21004-1L 1L PI-21004
21004-4L 4L
One-Step Lumitein™ UV Oriole™ and Flamingo™ Protein Gel Stains 21005-1L 1L PI-21005
21005-4L 4L
One-Step Blue is a registered trademark of Biotium. Lumitein and its related technologies are covered by US and international patents. SYPRO is a registered trademark of Molecular Probes, Inc. Oriole and Flamingo are trademarks of Bio-Rad Laboratories. Cubitainer is a registered trademark of Hedwin Corporation.

Reference Publications

Antonio Barreiro, Antoni Prenafeta, Gregori Bech-Sabat, Mercè Roca, Eva Perozo Mur, et al.

Preclinical evaluation of a COVID-19 vaccine candidate based on a recombinant RBD fusion heterodimer of SARS-CoV-2

bioRxiv 2021.11.22.469117;


Article Snippet:The purified antigen was then buffer exchanged by tangential flow filtration and filter sterilised. Purity and integrity were evaluated by SDS-PAGE with Bolt 4 to 12% Bis-Tris gels (Thermo Fisher, ref. NW04120BOX), stained with One-Step Blue Protein Gel Stain (Biotium, ref. 21003).”


Guisheng Zeng, Xiaoli Xu, Yee Jiun Kok, et al.

Cytochrome c regulates hyphal morphogenesis by interfering with cAMP-PKA signaling in Candida albicans

Cell Reports 42, 113473


Article Snippet: Highlight from the Chemicals, peptides, and recombinant proteins table “One-Step blue protein gel stain Biotium Cat#21003.”


Luo, Huacheng, et al.

HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia

Molecular cell vol. 82,4 (2022): 833-851.e11.

DOI: 10.1016/j.molcel.2022.01.014

Article Snippet: Highlight from the Chemicals, Peptides, and Recombinant Proteins table “BrUTP Biotium Cat#40026, One-Step Blue® Protein Gel Stain Biotium Cat#21003.”



Cell Reports

HHS Public