RPMI 1640 w/ L-Glutamine 500ml

RPMI 1640 w/ L-Glutamine 500ml

RPMI 1640 w/ L-Glutamine 500ml



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Classical Media

Many classical media are available with our partner Biowest portfolio. These include Dulbecco, Eagle, RPMI, Ham’s and many others. All of our products are suitable for cell culture applications and are made in modern ISO manufacturing facilities.

Product such as L0104 has proven to be compatible for cell lines obtained from ATCC, DSMZ and Riken.


[accordion title=”key features”]

· Our most popular RPMI formulation

· RPMI 1640 w/ L-Glutamine

· Sterile filtered; 12 months shelf life

· Proven success across wide spectrum of cell lines across several markets

· Widely accepted in key institutions like NUS, CSI (Singapore) and various R&D laboratories across S.E.A.

· All products are produced in ISO 9001:2008 and ISO 13485:2012 facilities.

· ** please inquiry for powdered formulation


[accordion title=”Formulation”] l0500_formula.pdf[/accordion]
[accordion title=”MSDS/Tech Sheet”]l0500_msds.pdf

[accordion title=”Product Brochure”]N/A[/accordion]

Reference Publications

Mostoufi-Afshar, S., Tabatabaei, M., & Ghahramani Seno, M. M.

Mycobacterium avium subsp. paratuberculosis induces differential cytosine methylation at miR-21 transcription start site region

Iranian journal of veterinary research, 19(4), 262–269

DOI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361596/

Article Snippet:The MAP cells were washed twice in plain RPMI1640 (Cat. No. L0500-500, Biowest, France) and resuspended in 1 ml of plain RPMI1640 by vortexing”


Cosín-Tomàs M, Senserrich J, Arumí-Planas M, Alquézar C, Pallàs M, Martín-Requero Á, Suñol C, Kaliman P, Sanfeliu C.

Role of Resveratrol and Selenium on Oxidative Stress and Expression of Antioxidant and Anti-Aging Genes in Immortalized Lymphocytes from Alzheimer’s Disease Patients

Nutrients. 2019; 11(8):1764.

DOI: https://doi.org/10.3390/nu11081764

Article Snippet:The cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 (Biowest, Nuaillé, France, #L0500), which contained 2 mM L-glutamine, supplemented with 10% fetal bovine serum”


Ciaffaglione V, Consoli V, Intagliata S, Marrazzo A, Romeo G, Pittalà V, Greish K, Vanella L, Floresta G, Rescifina A, et al.

Novel Tyrosine Kinase Inhibitors to Target Chronic Myeloid Leukemia

Molecules. 2022; 27(10):3220.

DOI: https://doi.org/10.3390/molecules27103220

Article Snippet:K562 cells (CCL-243 ATCC) were cultured in RPMI 1640 (L0500, Biowest, Riverside, MO, USA) supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin–streptomycin and incubated at 37 °C in 5% CO2.”


Mohammadi Abandansari R, Parsian H, Kazerouni F, Porbagher R, Zabihi E, et al.

Effect of Simultaneous Treatment with Royal Jelly and Doxorubicin on the Survival of the Prostate Cancer Cell Line (PC3): An In Vitro Study

Int J Cancer Manag. 2018;11(4):e13780.

DOI: https://doi.org/10.5812/ijcm.13780.

Article Snippet:PC3 cells were provided by the Pasture Institute (Tehran, Iran) and cultured in Roswell Park Memorial Institute (RPMI) 1640 culture medium (Biowest L0500 – 1000) containing 15% fetal bovine serum (FBS) (Biowest L1820 – 100) and penicillin-streptomycin 1% (Sigma P4333 – 100 mL).”


Niederauer, C., Nguyen, C., Wang-Henderson, M. et al.

Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions

Nat Commun 14, 4345 (2023).

DOI: https://doi.org/10.1038/s41467-023-40065-8

Article Snippet:Cell biology media and supplements used were DMEM without Phenol Red (12-917F), DMEM with Phenol Red (12-604F, Lonza), RPMI 1640 with Phenol Red (L0500-500, biowest), RPMI 1640 without Phenol Red (11835-030, gibco).”

Enrica E.K. Tan, Richard A. Hopkins, Chrissie K. Lim, Saumya S. Jamuar, Christina Ong, Koh C. Thoon, et al.

Dominant-negative NFKBIA mutation promotes IL-1β production causing hepatic disease with severe immunodeficiency

J Clin Invest. 2020;130(11):5817-5832.

DOI: https://doi.org/10.1172/JCI98882.

Article Snippet:Cells were differentiated in culture for 7 days with 20 ng/mL M-CSF (416-ML-50, R&D Systems) in RPMI 1640 media (L0500, Biowest) supplemented with 10% FBS …”


Teixido, Cristina & Marés, Rosó & Aracil, Miguel & Ramon Y Cajal, Santiago & Hernández-Losa, Javier.

Epithelial-Mesenchymal Transition Markers and HER3 Expression Are Predictors of Elisidepsin Treatment Response in Breast and Pancreatic Cancer Cell Lines

PloS one. 8. e53645.

DOI: 10.3390/ijms24032838

Article Snippet: “Both cell lines overexpress EpCAM [62,63] and the BxPC3 cell line overexpresses HER3 [64,65]. BxPC3 cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (L0500, Biowest) supplemented with 2 mM L-glutamine, 10% fetal bovine serum (FBS; F7524, Sigma-Aldrich), and 1% penicillin-streptomycin (L0022, Biowest, Berlin, Germany).”


Shiraz University



Research Article

Nature Communications

JCI Article

Research Gate