Reference Publications

Mohanasundaram P, Coelho-Rato LS, Modi MK, Urbanska M, Lautenschläger F, Cheng F, Eriksson JE

Cytoskeletal vimentin regulates cell size and autophagy through mTORC1 signaling

PLoS biology, 20(9), e3001737

DOI: https://doi.org/10.1371/journal.pbio.3001737

Article Snippet: “SV40-immortalised WT and Vim −/− MEFs were cultured in Dulbecco’s modified media (DMEM, Sigma #D6171) supplemented with 10% fetal bovine serum (FBS, Biowest #S1810), 2 mM of L-glutamine (Biowest #X0550), 100 U/ml of penicillin, and 100 μg/ml of streptomycin (Sigma #P0781) at 37°C in a 5% CO₂ incubator and passaged when they were about 80% confluent.”

Ali ASM, Berg J, Roehrs V, Wu D, Hackethal J, Braeuning A, Woelken L, Rauh C, Kurreck J

Xeno-Free 3D Bioprinted Liver Model for Hepatotoxicity Assessment

nternational Journal of Molecular Sciences. 2024; 25(3):1811

DOI: https://doi.org/10.3390/ijms25031811

Article Snippet: “In the direct approach, cells were directly cultured in different formulations of DMEM-F12 (L0090, Biowest) with L-glutamine (X0550, Biowest), HEPES (4-(2-Hydroxyethyl)-piperazin-1-ethansulfonsäure, L0180, Biowest), 1× P/S (L0022, Biowest), FCS (S-14-L, c.c.pro GmbH), non-essential amino acids (NEAA, Biowest)”

Magdalena Wolczyk, Remigiusz Serwa, Agata Kominek, Agata Klejman, Jacek Milek, Marta Chwałek, Laura Turos-Korgul, Agata Charzyńska, Michal Dabrowski, Magdalena Dziembowska, Tomasz Skorski, Katarzyna Piwocka, Paulina Podszywalow-Bartnicka,

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

iScience, Volume 26, Issue 4, 2023, 106543, ISSN 2589-0042

DOI: https://doi.org/10.1016/j.isci.2023.106543

Article Snippet: “murine cells in Iscove’s Modified Dulbecco’s Medium (Dulbecco, #30–2005) with 10% WEHI conditioned medium; either was supplemented with 100 U/ml penicillin, 100 μg/mL streptomycin (Biowest, #L0022), 2 mM L-Glutamine (Biowest, #X0550) and 10% FBS (v/v; Biowest, #S1810) as previously described”

Edo, Á., Calvo-Barreiro, L., Eixarch, H. et al

Therapeutic Effect of IL-21 Blockage by Gene Therapy in Experimental Autoimmune Encephalomyelitis

Neurotherapeutics 19, 1617–1633 (2022)

DOI: https://doi.org/10.1007/s13311-022-01279-8

Article Snippet: “In all proliferative assays, 2·10⁵ splenocytes/well were seeded in 96-well plates in 200 μL of proliferation medium (X-VIVO™ 15 medium (BE02-060F, Lonza, Basel, Switzerland) supplemented with 1% (v/v) L-glutamine (X0550, Biowest, Nuaillé, France), 0.4% (v/v) penicillin–streptomycin (L0022, Biowest), 0.1 M HEPES (H0887, Sigma-Aldrich), and 6 μM 2-β-mercaptoethanol (M3148, Sigma-Aldrich))”

Calvo-Barreiro L, Eixarch H, Ponce-Alonso M, et al.

A Commercial Probiotic Induces Tolerogenic and Reduces Pathogenic Responses in Experimental Autoimmune Encephalomyelitis

Cells. 2020 Apr;9(4):E906.

DOI: https://doi.org/10.3390/cells9040906

Article Snippet: “Splenocytes were seeded at 2 × 105
cells per well in X-VIVOTM 15 medium
(BE02-060F, Lonza, Basel, Switzerland) supplemented with 1% v/v L-glutamine (X0550, Biowest, Nuaillé,
France), 0.4% v/v penicillin-streptomycin (L0022, Biowest), 0.1 M HEPES (H0887, Sigma-Aldrich), and
6 µM 2-β-mercaptoethanol (M3148, Sigma-Aldrich) within 96-well plates”