Biowest L-Glutamine 100X, 200mM 100ml

Biowest L-Glutamine 100X, 200mM 100ml

Biowest L-Glutamine 100X, 200mM 100ml



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Media supplement

Media is often enriched with various amino acids, and vitamins to satisfy various cell culture applications.  All of our products are suitable for cell culture applications and are made in modern ISO manufacturing facilities. Products such as X0557 have proven to be successful in various R&D labs across Singapore and SEA.

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· Sterile filtered; 24 months expiry

· Proven success across a wide spectrum of cell lines across several markets

· Widely accepted in key universities like NUS, CSI ( Singapore) and various RND labs across SEA.

· All products are produced in ISO 9001:2008 and ISO 13485:2012 facilities.


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[accordion title=”MSDS/Tech Sheet”] x0550_msds.pdf

[accordion title=”Formula”] x0550_formula.pdf[/accordion]

Reference Publications

Mohanasundaram P, Coelho-Rato LS, Modi MK, Urbanska M, Lautenschläger F, Cheng F, Eriksson JE

Cytoskeletal vimentin regulates cell size and autophagy through mTORC1 signaling

PLoS biology20(9), e3001737


Article Snippet: “SV40-immortalised WT and Vim −/− MEFs were cultured in Dulbecco’s modified media (DMEM, Sigma #D6171) supplemented with 10% fetal bovine serum (FBS, Biowest #S1810), 2 mM of L-glutamine (Biowest #X0550), 100 U/ml of penicillin, and 100 μg/ml of streptomycin (Sigma #P0781) at 37°C in a 5% CO2 incubator and passaged when they were about 80% confluent.”


Ali ASM, Berg J, Roehrs V, Wu D, Hackethal J, Braeuning A, Woelken L, Rauh C, Kurreck J

Xeno-Free 3D Bioprinted Liver Model for Hepatotoxicity Assessment

nternational Journal of Molecular Sciences. 2024; 25(3):1811


Article Snippet: “In the direct approach, cells were directly cultured in different formulations of DMEM-F12 (L0090, Biowest) with L-glutamine (X0550, Biowest), HEPES (4-(2-Hydroxyethyl)-piperazin-1-ethansulfonsäure, L0180, Biowest), 1× P/S (L0022, Biowest), FCS (S-14-L, GmbH), non-essential amino acids (NEAA, Biowest)”


Magdalena Wolczyk, Remigiusz Serwa, Agata Kominek, Agata Klejman, Jacek Milek, Marta Chwałek, Laura Turos-Korgul, Agata Charzyńska, Michal Dabrowski, Magdalena Dziembowska, Tomasz Skorski, Katarzyna Piwocka, Paulina Podszywalow-Bartnicka,

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

iScience, Volume 26, Issue 4, 2023, 106543, ISSN 2589-0042


Article Snippet: “murine cells in Iscove’s Modified Dulbecco’s Medium (Dulbecco, #30–2005) with 10% WEHI conditioned medium; either was supplemented with 100 U/ml penicillin, 100 μg/mL streptomycin (Biowest, #L0022), 2 mM L-Glutamine (Biowest, #X0550) and 10% FBS (v/v; Biowest, #S1810) as previously described”


Edo, Á., Calvo-Barreiro, L., Eixarch, H. et al

Therapeutic Effect of IL-21 Blockage by Gene Therapy in Experimental Autoimmune Encephalomyelitis

Neurotherapeutics 19, 1617–1633 (2022)


Article Snippet: “In all proliferative assays, 2·105 splenocytes/well were seeded in 96-well plates in 200 μL of proliferation medium (X-VIVO™ 15 medium (BE02-060F, Lonza, Basel, Switzerland) supplemented with 1% (v/v) L-glutamine (X0550, Biowest, Nuaillé, France), 0.4% (v/v) penicillin–streptomycin (L0022, Biowest), 0.1 M HEPES (H0887, Sigma-Aldrich), and 6 μM 2-β-mercaptoethanol (M3148, Sigma-Aldrich))”


Calvo-Barreiro L, Eixarch H, Ponce-Alonso M, et al.

A Commercial Probiotic Induces Tolerogenic and Reduces Pathogenic Responses in Experimental Autoimmune Encephalomyelitis

Cells. 2020 Apr;9(4):E906.


Article Snippet: “Splenocytes were seeded at 2 × 105
cells per well in X-VIVOTM 15 medium
(BE02-060F, Lonza, Basel, Switzerland) supplemented with 1% v/v L-glutamine (X0550, Biowest, Nuaillé,
France), 0.4% v/v penicillin-streptomycin (L0022, Biowest), 0.1 M HEPES (H0887, Sigma-Aldrich), and
6 µM 2-β-mercaptoethanol (M3148, Sigma-Aldrich) within 96-well plates”


PLOS Biology

Molecular Science


Original Article

Cells Article