Media supplement
Media is often enriched with various amino acids, and vitamins to satisfy various cell culture applications. All of our products are suitable for cell culture applications and are made in modern ISO manufacturing facilities. Products such as X0557 have proven to be successful in various R&D labs across Singapore and SEA.
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· Sterile filtered; 24 months expiry
· Proven success across a wide spectrum of cell lines across several markets
· Widely accepted in key universities like NUS, CSI ( Singapore) and various RND labs across SEA.
· All products are produced in ISO 9001:2008 and ISO 13485:2012 facilities.
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[accordion title=”MSDS/Tech Sheet”] x0550_msds.pdf
x0550_techsheet.pdf[/accordion]
[accordion title=”Formula”] x0550_formula.pdf[/accordion]
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Reference Publications
Mohanasundaram P, Coelho-Rato LS, Modi MK, Urbanska M, Lautenschläger F, Cheng F, Eriksson JE
Cytoskeletal vimentin regulates cell size and autophagy through mTORC1 signaling
PLoS biology, 20(9), e3001737
DOI: https://doi.org/10.1371/journal.pbio.3001737
Article Snippet: “SV40-immortalised WT and Vim −/− MEFs were cultured in Dulbecco’s modified media (DMEM, Sigma #D6171) supplemented with 10% fetal bovine serum (FBS, Biowest #S1810), 2 mM of L-glutamine (Biowest #X0550), 100 U/ml of penicillin, and 100 μg/ml of streptomycin (Sigma #P0781) at 37°C in a 5% CO2 incubator and passaged when they were about 80% confluent.”
Ali ASM, Berg J, Roehrs V, Wu D, Hackethal J, Braeuning A, Woelken L, Rauh C, Kurreck J
Xeno-Free 3D Bioprinted Liver Model for Hepatotoxicity Assessment
nternational Journal of Molecular Sciences. 2024; 25(3):1811
DOI: https://doi.org/10.3390/ijms25031811
Article Snippet: “In the direct approach, cells were directly cultured in different formulations of DMEM-F12 (L0090, Biowest) with L-glutamine (X0550, Biowest), HEPES (4-(2-Hydroxyethyl)-piperazin-1-ethansulfonsäure, L0180, Biowest), 1× P/S (L0022, Biowest), FCS (S-14-L, c.c.pro GmbH), non-essential amino acids (NEAA, Biowest)”
Magdalena Wolczyk, Remigiusz Serwa, Agata Kominek, Agata Klejman, Jacek Milek, Marta Chwałek, Laura Turos-Korgul, Agata Charzyńska, Michal Dabrowski, Magdalena Dziembowska, Tomasz Skorski, Katarzyna Piwocka, Paulina Podszywalow-Bartnicka,
TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment
iScience, Volume 26, Issue 4, 2023, 106543, ISSN 2589-0042
DOI: https://doi.org/10.1016/j.isci.2023.106543
Article Snippet: “murine cells in Iscove’s Modified Dulbecco’s Medium (Dulbecco, #30–2005) with 10% WEHI conditioned medium; either was supplemented with 100 U/ml penicillin, 100 μg/mL streptomycin (Biowest, #L0022), 2 mM L-Glutamine (Biowest, #X0550) and 10% FBS (v/v; Biowest, #S1810) as previously described”
Edo, Á., Calvo-Barreiro, L., Eixarch, H. et al
Therapeutic Effect of IL-21 Blockage by Gene Therapy in Experimental Autoimmune Encephalomyelitis
Neurotherapeutics 19, 1617–1633 (2022)
DOI: https://doi.org/10.1007/s13311-022-01279-8
Article Snippet: “In all proliferative assays, 2·105 splenocytes/well were seeded in 96-well plates in 200 μL of proliferation medium (X-VIVO™ 15 medium (BE02-060F, Lonza, Basel, Switzerland) supplemented with 1% (v/v) L-glutamine (X0550, Biowest, Nuaillé, France), 0.4% (v/v) penicillin–streptomycin (L0022, Biowest), 0.1 M HEPES (H0887, Sigma-Aldrich), and 6 μM 2-β-mercaptoethanol (M3148, Sigma-Aldrich))”
Calvo-Barreiro L, Eixarch H, Ponce-Alonso M, et al.
A Commercial Probiotic Induces Tolerogenic and Reduces Pathogenic Responses in Experimental Autoimmune Encephalomyelitis
Cells. 2020 Apr;9(4):E906.
DOI: https://doi.org/10.3390/cells9040906
Article Snippet: “Splenocytes were seeded at 2 × 105
cells per well in X-VIVOTM 15 medium
(BE02-060F, Lonza, Basel, Switzerland) supplemented with 1% v/v L-glutamine (X0550, Biowest, Nuaillé,
France), 0.4% v/v penicillin-streptomycin (L0022, Biowest), 0.1 M HEPES (H0887, Sigma-Aldrich), and
6 µM 2-β-mercaptoethanol (M3148, Sigma-Aldrich) within 96-well plates”